Literature summary for 4.2.3.12 extracted from

Burgisser, D.M.; Th�ny, B.; Redweik, U.; Hunziker, P.; Heizmann, C.W.; Blau, N.
Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity (1994), Eur. J. Biochem., 219, 497-502.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli	Rattus norvegicus
expressed as maltose-binding-6-pyruvoyl-tetrahydropterin-synthase fusion protein	Homo sapiens
expressed as maltose-binding-6-pyruvoyl-tetrahydropterin-synthase fusion protein	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-Vinylpyridine	95% inactivation under non-denaturating and non-reducing conditions	Homo sapiens
4-Vinylpyridine	80% inactivation under non-denaturating and non-reducing conditions	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	wild-type enzyme	Rattus norvegicus
0.008	-	7,8-dihydroneopterin triphosphate	pH 7.4, 37ºC	Rattus norvegicus
0.0085	-	7,8-dihydroneopterin triphosphate	pH 7.4, 37ºC	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15899	-	3 * 15899, electron-ionization mass spectrometry	Rattus norvegicus
16000	-	x * 16000, SDS-PAGE and electrospray ionization-mass spectometry	Homo sapiens
16254	-	6 * 16254, electron-ionization mass spectrometry	Homo sapiens
47000	-	3 * 47000, SDS-PAGE	Homo sapiens
97000	-	6 * 97000, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	Rattus norvegicus	-	6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate	-	?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	Homo sapiens	rate-limiting enzyme in the synthesis of human tetrahydrobiopterin	6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography, gel filtration , SDS-PAGE	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens
additional information	-	-	Rattus norvegicus
0.077	-	-	Homo sapiens
0.2766	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	-	Homo sapiens	6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate	-	?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	-	Rattus norvegicus	6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate	-	?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	rate-limiting enzyme in the synthesis of human tetrahydrobiopterin	Homo sapiens	6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate	-	?

Subunits

Subunits	Comment	Organism
?	-	Rattus norvegicus
?	x * 16000, SDS-PAGE and electrospray ionization-mass spectometry	Homo sapiens
hexamer	-	Rattus norvegicus
hexamer	6 * 97000, SDS-PAGE	Homo sapiens
hexamer	6 * 16254, electron-ionization mass spectrometry	Homo sapiens
trimer	3 * 47000, SDS-PAGE	Homo sapiens
trimer	3 * 15899, electron-ionization mass spectrometry	Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	stable	Homo sapiens
80	-	stable	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	dihydroneopterin triphosphate	0.277 mU/min/mg	Rattus norvegicus
additional information	-	dihydroneopterin triphosphate	0.080 mU/min/mg	Homo sapiens

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Release 2023.1 (January 2023)