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Literature summary for 4.2.3.12 extracted from
- Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from human pituitary gland (1992), Enzyme, 46, 287-298.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NADPH is not necessary for activity | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| stable at all steps of purification | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | L-monapterin does not inhibit activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens |  |
| 0.01 | - |
7,8-dihydroneopterin triphosphate | pH 7.4, 37ºC, shows positive cooperativity | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | necessary for activity | Homo sapiens | |
| Mg2+ | required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
4 * 16500, SDS-PAGE | Homo sapiens |
| 16500 | - |
x * 16500, SDS-PAGE | Homo sapiens |
| 68000 | - |
gel filtration | Homo sapiens |
| 68000 | - |
gel filtration | Salmo salar |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | Drosophila melanogaster | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | Homo sapiens | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | Salmo salar | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Salmo salar | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sephadex A50 column | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| head | - |
Drosophila melanogaster | - |
| hypophysis | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Salmo salar | - |
| pituitary gland | heat instability, it is present in the anterior pituitary endothelial cells but not in posterior pituitary | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | - |
Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | - |
Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | - |
Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Salmo salar |
| ? | - |
Drosophila melanogaster |
| ? | x * 16500, SDS-PAGE | Homo sapiens |
| tetramer | - |
Salmo salar |
| tetramer | 4 * 16500, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Drosophila melanogaster |
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Homo sapiens |
| additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Salmo salar |
| additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
| 7.5 | - |
- |
Salmo salar |
| 7.5 | - |
- |
Drosophila melanogaster |
| 7.5 | - |
in Tris-HCl 100 mM | Homo sapiens |
| 7.5 | - |
in Tris-HCl 100 mM | Salmo salar |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | required | Homo sapiens | |
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