Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.12 extracted from

  • Thöny, B.; Leimbacher, W.; Blau, N.; Heizmann, C.W.; Burgisser, D.
    Human liver 6-pyruvoyl-tetrahydropterin synthase: expression of the cDNA, purification and preliminary characterization of the recombinant protein (1993), Adv. Exp. Med. Biol., 338, 187-190.
    View publication on PubMed
Search for more literature for 4.2.3.12

Application

Application Comment Organism
medicine lack of tetrahydrobiopterin leads to hyperphenylalaninemia and a deficiency of biogenic amine neurotransmitters such as dopamine and serotonin and severe progressive mental retardation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli Rattus norvegicus
expression in Escherichia coli Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Rattus norvegicus
Zn2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 5 * 16000, SDS-PAGE Homo sapiens
16000
-
 x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
16257
-
 5 * 16257, electrospray ionization-mass spectrometry Homo sapiens
83000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Homo sapiens the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Rattus norvegicus the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
 cloned in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, gel filtration, SDS-PAGE Homo sapiens
affinity chromatography, gel filtration, SDS-PAGE Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0574
-
-
 Homo sapiens
57.4
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Rattus norvegicus 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway Rattus norvegicus 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

Subunits Comment Organism
?
-
 Rattus norvegicus
? x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
pentamer 5 * 16000, SDS-PAGE Homo sapiens
pentamer 5 * 16257, electrospray ionization-mass spectrometry Homo sapiens