Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.117 extracted from

  • Ruan, J.X.; Li, J.X.; Fang, X.; Wang, L.J.; Hu, W.L.; Chen, X.Y.; Yang, C.Q.
    Isolation and characterization of three new monoterpene synthases from Artemisia annua (2016), Front. Plant Sci., 7, 638 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
AaTPS5, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3) Artemisia annua

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01974
-
geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, Mg2+ is the preferred divalent cation Artemisia annua
Mn2+ low activity Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Artemisia annua stereochemistry is not specified in the publication camphene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Artemisia annua A0A068L7N8 cf. EC 4.2.3.116
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Artemisia annua

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence low enzyme content Artemisia annua
-
leaf transcriptof AaTPS5 is more abundant in young leaves compared to immature leaves, no enzyme in mature leaves Artemisia annua
-
root low to moderate enzyme content Artemisia annua
-
stem low enzyme content Artemisia annua
-
trichome glandular Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate stereochemistry is not specified in the publication Artemisia annua camphene + diphosphate
-
?
additional information GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products Artemisia annua ?
-
?

Synonyms

Synonyms Comment Organism
AaTPS5
-
Artemisia annua
More cf. EC 4.2.3.116 Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.49
-
geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Artemisia annua

Expression

Organism Comment Expression
Artemisia annua AaTPS5 is upregulated after mechanical wounding and by treatment with 5 mM salicylic acid, 50 mM methyl jasmonate, or 100 mM gibberellin up

General Information

General Information Comment Organism
evolution the enzyme TPS5 belongs to the TPS superfamily, subclade TPS-b Artemisia annua
additional information analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
75.5
-
geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua