Literature summary for 4.2.3.117 extracted from

Tsaballa, A.; Nikolaidis, A.; Trikka, F.; Ignea, C.; Kampranis, S.; Makris, A.; Argiriou, A.
Use of the de novo transcriptome analysis of silver-leaf nightshade (Solanum elaeagnifolium) to identify gene expression changes associated with wounding and terpene biosynthesis (2015), BMC Genomics, 16, 504 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene cl9841, the TPS gene identified, transcript cl9841, is a cluster of 9 sequences but only one is expressed in leaves and flowers. The protein shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, DNA and amino acid sequence determination and analysis, recombinant coexpression with copalyl diphosphate synthase from Salvia pomifera and Saccharomyces cerevisiae ERG20 mutant F96C producing GGPP in Saccharomyces cerevisiae strain AM 238	Solanum elaeagnifolium

Cloned (Comment)

Organism

gene cl9841, the TPS gene identified, transcript cl9841, is a cluster of 9 sequences but only one is expressed in leaves and flowers. The protein shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, DNA and amino acid sequence determination and analysis, recombinant coexpression with copalyl diphosphate synthase from Salvia pomifera and Saccharomyces cerevisiae ERG20 mutant F96C producing GGPP in Saccharomyces cerevisiae strain AM 238

Solanum elaeagnifolium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate	Solanum elaeagnifolium	-	(-)-camphene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Solanum elaeagnifolium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Solanum elaeagnifolium	(-)-camphene + diphosphate	-	?
additional information	gene cl9841 is an active monoterpene synthase enzyme producing a range of monoterpenes with the most prominent being camphene (52.55%), beta-myrcene (11.01%), and limonene (10.44%) and several minor additional compounds. GC-MS qualitative and semi-quantitative analysis of terpene compounds, overview	Solanum elaeagnifolium	?	-	?

Synonyms

Synonyms	Comment	Organism
cl9841	-	Solanum elaeagnifolium

General Information

General Information	Comment	Organism
evolution	the enzyme shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, suggesting it to have (-)-camphene synthase activity	Solanum elaeagnifolium