Cloned (Comment) | Organism |
---|---|
AaTPS5, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3) | Artemisia annua |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01974 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, Mg2+ is the preferred divalent cation | Artemisia annua | |
Mn2+ | low activity | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Artemisia annua | stereochemistry is not specified in the publication | camphene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | A0A068L7N8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
inflorescence | low enzyme content | Artemisia annua | - |
leaf | transcript of AaTPS5 is more abundant in young leaves compared to immature leaves, no enzyme in mature leaves | Artemisia annua | - |
root | low to moderate enzyme content | Artemisia annua | - |
stem | low enzyme content | Artemisia annua | - |
trichome | glandular | Artemisia annua | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | stereochemistry is not specified in the publication | Artemisia annua | camphene + diphosphate | - |
? | |
additional information | GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products | Artemisia annua | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AaTPS5 | - |
Artemisia annua |
More | cf. EC 4.2.3.117 | Artemisia annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Artemisia annua |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.49 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Artemisia annua |
Organism | Comment | Expression |
---|---|---|
Artemisia annua | AaTPS5 is upregulated after mechanical wounding and by treatment with 5 mM salicylic acid, 50 mM methyl jasmonate, or 100 mM gibberellin | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme TPS5 belongs to the TPS superfamily, subclade TPS-b | Artemisia annua |
additional information | analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview | Artemisia annua |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75.5 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |