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Literature summary for 4.2.3.114 extracted from
- Rice terpene synthase 24 (OsTPS24) encodes a jasmonate-responsive monoterpene synthase that produces an antibacterial gamma-terpinene against rice pathogen (2016), J. Plant Physiol., 191, 120-126 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene OsTPS24, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, functional recombinant expression of His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence in Escherichia coli, expression of GFP-tagged enzyme in Oryza sativa protoplasts chloroplasts | Oryza sativa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | the enzyme has a chloroplast transit sequence | Oryza sativa | 9507 | - |
| additional information | in general, monoterpene synthases contain a transit peptide for translocation to plastids and sesquiterpene synthases are localized in the cytoplasm | Oryza sativa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | Oryza sativa | - |
gamma-terpinene + diphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
cv. Nipponbare | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence from Escherichia coli by nickel affinity chromatography and dialysis | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Oryza sativa | gamma-terpinene + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67700, about, sequence calculation | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Os04g27790 | locus name | Oryza sativa |
| OsTPS24 | - |
Oryza sativa |
| terpene synthase 24 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryza sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oryza sativa | the enzyme encoded by gene OsTPS24 is highly induced about 20fold by jasmonate, expression pattern, overview | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme OsTPS24 is localized in chloroplasts and produced the monoterpene, gamma-terpinene. The amount of gamma-terpinene increased after jasmonate treatment. gamma-Terpinene has significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo), but it does not show significant antifungal activity against Magnaporthe oryzae. The antibacterial activity of gamma-terpinene against Xoo is caused by damage to bacterial cell membranes. These results suggest that gamma-terpinene plays an important role in jasmonate-induced resistance against Xoo, and that it functions as an antibacterial compound in rice plants | Oryza sativa |
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Release 2023.1 (January 2023)
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