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Literature summary for 4.2.3.114 extracted from
- Purification and characterization of the monoterpene cyclase gamma-terpinene synthase from Thymus vulgaris (1991), Arch. Biochem. Biophys., 286, 511-517.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | - |
Thymus vulgaris |  |
| benzyl bromide | - |
Thymus vulgaris | |
| diethyl dicarbonate | - |
Thymus vulgaris | |
| diphosphate | - |
Thymus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
geranyl diphosphate | pH 6.8, 30°C | Thymus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Thymus vulgaris | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required. Optimum concentration 20 mM | Thymus vulgaris | |
| Mn2+ | or Mg2+, required. 50% of the efficiency with Mg2+ | Thymus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Thymus vulgaris |
| 96000 | - |
gel fitlration | Thymus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thymus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thymus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 149 | - |
pH 6.8, 30°C | Thymus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Thymus vulgaris | gamma-terpinene + diphosphate | products are about 64% gamma-terpinen, 10% alpha-thujene, 4% myrcene, 4% alpha-terpinene, 2% limonene, 3% linalool, 2% terpinen-4-ol, 3% alpha-terpineol | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Thymus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Thymus vulgaris |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thymus vulgaris | isoelectric focusing | - |
4.9 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
pH 6.8, 30°C | Thymus vulgaris | 4-hydroxymercuribenzoate | |
| 0.01 | - |
pH 7.0, 30°C | Thymus vulgaris | diethyl dicarbonate | |
| 0.5 | - |
pH 6.8, 30°C | Thymus vulgaris | diphosphate | |
| 2 | - |
pH 6.0, 30°C | Thymus vulgaris | benzyl bromide | |
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Release 2023.1 (January 2023)
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