General Stability | Organism |
---|---|
addition of either 20% glycerol or 1 mM substrate does not significantly improve stability | Thymus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, complete inactivation | Thymus vulgaris | |
additional information | not inhibitory: gamma-terpinene, p-cymene, thymol | Thymus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
neryl diphosphate | pH 6.6, 30°C | Thymus vulgaris | |
0.014 | - |
geranyl diphosphate | pH 6.6, 30°C | Thymus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, required, Mg2+ is preferred over Mn2+ | Thymus vulgaris | |
Mn2+ | or Mg2+, required, about 60% of the efficiency qith Mg2+ | Thymus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
gel filtration | Thymus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thymus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Thymus vulgaris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranyl diphosphate = gamma-terpinene + diphosphate | mechanism involves the cyclizazion of the acyclic precursor, loss of a proton from C5 to form the delta4 double bond, and a 1,2-hydride shift fom C4 to C8 to give gamma-terpinene | Thymus vulgaris |
Storage Stability | Organism |
---|---|
0°C, 40 mM sodium phosphate, 5 mM MgCl2, 0.5 mM dithioerythritol, loss of 60% of activity within 1 week | Thymus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Thymus vulgaris | gamma-terpinene + diphosphate | - |
? | |
neryl diphosphate | - |
Thymus vulgaris | gamma-terpinene + diphosphate | 75% gamma-terpinene, 20% alpha-thujene, 5% alpha-terpineol, partially purified enzyme | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Thymus vulgaris |