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Literature summary for 4.2.3.114 extracted from

  • Lucker, J.; El Tamer, M.K.; Schwab, W.; Verstappen, F.W.; van der Plas, L.H.; Bouwmeester, H.J.; Verhoeven, H.A.
    Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases (2000), Eur. J. Biochem., 269, 3160-3171.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrus limon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
geranyl diphosphate pH 7.0, 30°C Citrus limon

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, Mn2+ is preferred over Mg2+ Citrus limon
Mn2+ or Mg2+, Mn2+ is preferred over Mg2+, optimum concentration 0.6 mM Citrus limon

Organism

Organism UniProt Comment Textmining
Citrus limon Q8L5K4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit glants of fruit lavedo and fruit peel Citrus limon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
Citrus limon gamma-terpinene + diphosphate products are 71.4% gamma-terpinene, with lower amounts of limonene (9.1%), alpha-pinene (5.6%), beta-pinene (4.7%), alpha-terpinolene (3.7%), alpha-thujene (2.5%), alpha-terpinene (1.7%), myrcene (0.9%), sabinene (0.4%) and a trace of p-cymene ?