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Literature summary for 4.2.3.108 extracted from

  • Yahyaa, M.; Matsuba, Y.; Brandt, W.; Doron-Faigenboim, A.; Bar, E.; McClain, A.; Davidovich-Rikanati, R.; Lewinsohn, E.; Pichersky, E.; Ibdah, M.
    Identification, functional characterization, and evolution of terpene synthases from a basal dicot (2015), Plant Physiol., 169, 1683-1697 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Laurus nobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the putative amino-terminal extension of 45 amino acids of the LnTPS1 upstream of the RRx8W motif and sequence analysis predicts a plastidic localization for the enzyme Laurus nobilis 9507
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Laurus nobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + H2O Laurus nobilis
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1,8-cineole + diphosphate
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?

Organism

Organism UniProt Comment Textmining
Laurus nobilis A0A0H4U5M2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Laurus nobilis

Source Tissue

Source Tissue Comment Organism Textmining
flower especially female flowers Laurus nobilis
-
fruit ripe Laurus nobilis
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leaf high enzyme content, fresh Laurus nobilis leaves, the monoterpene 1,8-cineole is the main volatile compound, and alpha-terpinyl acetate, terpinene-4-ol, alpha- and beta-pinene, sabinene, and linalool are reported to occur in appreciable levels Laurus nobilis
-
additional information volatile profiles in leaves, flowers, fruits, and roots in male and female Laurus nobilis plants, overview Laurus nobilis
-
root
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Laurus nobilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + H2O
-
Laurus nobilis 1,8-cineole + diphosphate
-
?
additional information with geranyl pyrophosphate (GPP) as a substrate, LnTPS1 catalyzes the formation of mostly 1,8-cineole, with alpha-thujene, alpha-pinene, beta-pinene, alpha-terpinene, gamma-terpinene, alpha-terpinolene, and a few other monoterpenes also produced, as detected by GC-MS analysis Laurus nobilis ?
-
?

Subunits

Subunits Comment Organism
? x * 67400, about, sequence calculation Laurus nobilis

Synonyms

Synonyms Comment Organism
LnTPS1
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Laurus nobilis
TPS1
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Laurus nobilis

General Information

General Information Comment Organism
evolution the monoterpene synthase belongs to the terpene synthases superfamily, TPS-b clade Laurus nobilis
physiological function fresh Laurus nobilis leaves, the monoterpene 1,8-cineole is the main volatile compound, and alpha-terpinyl acetate, terpinene-4-ol, alpha- and beta-pinene, sabinene, and linalool are reported to occur in appreciable levels. The 1,8-cineole content is also higher in femal flowers, overview Laurus nobilis