Cloned (Comment) | Organism |
---|---|
gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Laurus nobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the putative amino-terminal extension of 45 amino acids of the LnTPS1 upstream of the RRx8W motif and sequence analysis predicts a plastidic localization for the enzyme | Laurus nobilis | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Laurus nobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | Laurus nobilis | - |
1,8-cineole + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laurus nobilis | A0A0H4U5M2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Laurus nobilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | especially female flowers | Laurus nobilis | - |
fruit | ripe | Laurus nobilis | - |
leaf | high enzyme content, fresh Laurus nobilis leaves, the monoterpene 1,8-cineole is the main volatile compound, and alpha-terpinyl acetate, terpinene-4-ol, alpha- and beta-pinene, sabinene, and linalool are reported to occur in appreciable levels | Laurus nobilis | - |
additional information | volatile profiles in leaves, flowers, fruits, and roots in male and female Laurus nobilis plants, overview | Laurus nobilis | - |
root | - |
Laurus nobilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | - |
Laurus nobilis | 1,8-cineole + diphosphate | - |
? | |
additional information | with geranyl pyrophosphate (GPP) as a substrate, LnTPS1 catalyzes the formation of mostly 1,8-cineole, with alpha-thujene, alpha-pinene, beta-pinene, alpha-terpinene, gamma-terpinene, alpha-terpinolene, and a few other monoterpenes also produced, as detected by GC-MS analysis | Laurus nobilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67400, about, sequence calculation | Laurus nobilis |
Synonyms | Comment | Organism |
---|---|---|
LnTPS1 | - |
Laurus nobilis |
TPS1 | - |
Laurus nobilis |
General Information | Comment | Organism |
---|---|---|
evolution | the monoterpene synthase belongs to the terpene synthases superfamily, TPS-b clade | Laurus nobilis |
physiological function | fresh Laurus nobilis leaves, the monoterpene 1,8-cineole is the main volatile compound, and alpha-terpinyl acetate, terpinene-4-ol, alpha- and beta-pinene, sabinene, and linalool are reported to occur in appreciable levels. The 1,8-cineole content is also higher in femal flowers, overview | Laurus nobilis |