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Literature summary for 4.2.3.108 extracted from

  • Chen, F.; Ro, D.K.; Petri, J.; Gershenzon, J.; Bohlmann, J.; Pichersky, E.; Tholl, D.
    Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole (2004), Plant Physiol., 135, 1956-1966.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.108

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, absolutely required. Fully active at 40 mM Arabidopsis thaliana
Mn2+ or Mg2+, absolutely required. Fully active at 1 mM Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P0DI76
-
-
Arabidopsis thaliana P0DI77 isoform 2
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermis promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
root promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
stele promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + H2O
-
 Arabidopsis thaliana 1,8-cineole + diphosphate 52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol ?
additional information no substrate: farnesyl diphosphate Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana