Literature summary for 4.2.3.108 extracted from

Kampranis, S.C.; Ioannidis, D.; Purvis, A.; Mahrez, W.; Ninga, E.; Katerelos, N.A.; Anssour, S.; Dunwell, J.M.; Degenhardt, J.; Makris, A.M.; Goodenough, P.W.; Johnson, C.B.
Rational conversion of substrate and product specificity in a Salvia monoterpene synthase: structural insights into the evolution of terpene synthase function (2007), Plant Cell, 19, 1994-2005.

Search for more literature for 4.2.3.108

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.95 A resolution, space group C2221. alpha-Helical protein comprised of 23 alpha-helices and eight 3/10 helices	Salvia fruticosa

Protein Variants

Protein Variants	Comment	Organism
additional information	conversion of CinS1 to a sabinene synthase based on an amino acid comparison of the enzyme with Salvia officinalis SabS1 and Salvia pomifera SabS1	Salvia fruticosa
N338A	products are 11.7% alpha-pinene, 33.1% sabinene, 33.2% beta-pinene, 16.9% myrcene, 5.1% limonene	Salvia fruticosa
N338C	products are 16.1% alpha-pinene, 41% sabinene, 32.1% beta-pinene, 4.7% myrcene, 6.1% limonene	Salvia fruticosa
N338I	products are 6.5% alpha-pinene, 48.3% sabinene, 8.2% myrcene, 37% limonene	Salvia fruticosa
N338I/A339T	products are 5.4% alpha-pinene, 62.1% sabinene, 6.4% myrcene, 26.1% limonene	Salvia fruticosa
N338I/A339T/G447S	products are 5.7% alpha-pinene, 59.6% sabinene, 5.0% myrcene, 29.7% limonene	Salvia fruticosa
N338I/A339T/G447S/I449P/P450T	products are 86.8% sabinene, 5.5% myrcene, 7.7% limonene	Salvia fruticosa
N338L	products are 4.7% alpha-pinene, 5.6% sabinene, 13.2% beta-pinene, 27.2% myrcene, 49.3% limonene	Salvia fruticosa
N338S	products are 9.8% alpha-pinene, 34.5% sabinene, 34.2% beta-pinene, 6.8% myrcene, 2.6% limonene, 5.6% alpha-cineole, 6.5% alpha-terpineol	Salvia fruticosa
N338V	products are 61.2% sabinene, 8.0% myrcene, 30.8% limonene	Salvia fruticosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0119	-	geranyl diphosphate	mutant N338A, pH 7.0, 22°C	Salvia fruticosa
0.0129	-	geranyl diphosphate	mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C	Salvia fruticosa
0.0144	-	geranyl diphosphate	mutant N338S, pH 7.0, 22°C	Salvia fruticosa
0.0265	-	geranyl diphosphate	mutant N338L, pH 7.0, 22°C	Salvia fruticosa
0.055	-	geranyl diphosphate	mutant N338V, pH 7.0, 22°C	Salvia fruticosa
0.0566	-	geranyl diphosphate	mutant N338I, pH 7.0, 22°C	Salvia fruticosa
0.0654	-	geranyl diphosphate	wild-type, pH 7.0, 22°C	Salvia fruticosa
0.0955	-	geranyl diphosphate	mutant N338I/A339T/G447S, pH 7.0, 22°C	Salvia fruticosa
0.136	-	geranyl diphosphate	mutant N338I/A339T, pH 7.0, 22°C	Salvia fruticosa
0.201	-	geranyl diphosphate	mutant N338C, pH 7.0, 22°C	Salvia fruticosa

Organism

Organism	UniProt	Comment	Textmining
Salvia fruticosa	A6XH05	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + H2O	-	Salvia fruticosa	1,8-cineole + diphosphate	72.4% 1,8-cineole, 7.1% alpha-terpineol, 9.1% beta-pinene, 4.6% alpha-pinene, 3.6% sabinene, 2.2% myrcene, and <1% limonene	?

Synonyms

Synonyms	Comment	Organism
SabS1	-	Salvia fruticosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0043	-	geranyl diphosphate	mutant N338I/A339T/G447S, pH 7.0, 22°C	Salvia fruticosa
0.0043	-	geranyl diphosphate	mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C	Salvia fruticosa
0.009	-	geranyl diphosphate	mutant N338L, pH 7.0, 22°C	Salvia fruticosa
0.012	-	geranyl diphosphate	mutant N338V, pH 7.0, 22°C	Salvia fruticosa
0.022	-	geranyl diphosphate	mutant N338S, pH 7.0, 22°C	Salvia fruticosa
0.024	-	geranyl diphosphate	mutant N338I/A339T, pH 7.0, 22°C	Salvia fruticosa
0.052	-	geranyl diphosphate	mutant N338C, pH 7.0, 22°C	Salvia fruticosa
0.053	-	geranyl diphosphate	wild-type, pH 7.0, 22°C	Salvia fruticosa
0.069	-	geranyl diphosphate	mutant N338A, pH 7.0, 22°C	Salvia fruticosa
0.096	-	geranyl diphosphate	mutant N338I, pH 7.0, 22°C	Salvia fruticosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.045	-	geranyl diphosphate	mutant N338I/A339T/G447S, pH 7.0, 22°C	Salvia fruticosa
0.177	-	geranyl diphosphate	mutant N338I/A339T, pH 7.0, 22°C	Salvia fruticosa
0.22	-	geranyl diphosphate	mutant N338V, pH 7.0, 22°C	Salvia fruticosa
0.26	-	geranyl diphosphate	mutant N338C, pH 7.0, 22°C	Salvia fruticosa
0.33	-	geranyl diphosphate	mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C	Salvia fruticosa
0.34	-	geranyl diphosphate	mutant N338L, pH 7.0, 22°C	Salvia fruticosa
0.81	-	geranyl diphosphate	wild-type, pH 7.0, 22°C	Salvia fruticosa
1.53	-	geranyl diphosphate	mutant N338S, pH 7.0, 22°C	Salvia fruticosa
1.69	-	geranyl diphosphate	mutant N338I, pH 7.0, 22°C	Salvia fruticosa
5.79	-	geranyl diphosphate	mutant N338A, pH 7.0, 22°C	Salvia fruticosa

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Release 2023.1 (January 2023)