Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methyl jasmonate | protein is detected at significantly higher levels in susceptible trees compared to resistant trees at day 7 after methyl jasmonate treatment | Picea sitchensis |
Cloned (Comment) | Organism |
---|---|
- |
Picea sitchensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | isoform 3car3 has an extremely low activity | Picea sitchensis | |
0.00688 | - |
geranyl diphosphate | pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters | Picea sitchensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea sitchensis | F1CKI6 | isoform TPS-3car1, expressed both in trees susceptible and resistant to white pine weevil | - |
Picea sitchensis | F1CKI8 | isoform TPS-3car2, only expressed in trees resistant to white pine weevil | - |
Picea sitchensis | F1CKI9 | isoform TPS-3car3, only expressed in trees susceptilble to white pine weevil | - |
Purification (Comment) | Organism |
---|---|
- |
Picea sitchensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Picea sitchensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Picea sitchensis | (+)-car-3-ene + diphosphate | major product, plus some terpinolene | ? | |
additional information | poor substrate: neryl diphosphate. No substrates: farnesyl diphosphate, geranylgeranyl diphosphate | Picea sitchensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS-3car1 | - |
Picea sitchensis |
TPS-3car2 | - |
Picea sitchensis |
TPS-3car3 | - |
Picea sitchensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.21 | - |
geranyl diphosphate | pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters | Picea sitchensis |
Organism | Comment | Expression |
---|---|---|
Picea sitchensis | transcript levels are increased following methyl jasmonate treatment, with transcripts detected at significantly higher abundance in resistant trees compared to susceptible trees | up |
General Information | Comment | Organism |
---|---|---|
physiological function | (+)-3-carene is associated with resistance of Sitka spruce to white pine weevil, Pissodes strobi. Variation of the (+)-3-carene phenotype is controlled by copy number variation of TPS-3car genes, variation of gene and protein expression, and variation in catalytic efficiencies | Picea sitchensis |