Literature summary for 4.2.2.B7 extracted from

Wang, D.; Aarstad, O.A.; Li, J.; McKee, L.S.; Saetrom, G.I.; Vyas, A.; Srivastava, V.; Aachmann, F.L.; Bulone, V.; Hsieh, Y.S.
Preparation of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and guluronic acid rich alginate using a unique exo-alginate lyase from Thalassotalea crassostreae (2018), J. Agric. Food Chem., 66, 1435-1443 .

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Application

Application	Comment	Organism
synthesis	production of highly pure 4-deoxy-L-erythro-5-hexoseulose uronic acid using 0.78 mg of TcAlg1 per ml substrate solution (2% w/v of sodium alginate) during 12 h, overall yield is 28%	Thalassotalea crassostreae

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thalassotalea crassostreae

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure. Features around the active site indicate that Alg1 shows substrate preference for poly M units in the alginate molecule	Thalassotalea crassostreae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
9.2	-	poly(beta-(1,4)-D-mannuronate)	pH 7.0, 30°C	Thalassotalea crassostreae
23.9	-	poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)	pH 7.0, 30°C	Thalassotalea crassostreae

Organism

Organism	UniProt	Comment	Textmining
Thalassotalea crassostreae	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	sequence contains a predicted signal peptide	Thalassotalea crassostreae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme shows preference for poly(beta-(1,4)-D-mannuronate). The residual alginate fractions are enriched in guluronic acid	Thalassotalea crassostreae	?	-	?
poly(alpha-(1,4)-L-guluronate)	-	Thalassotalea crassostreae	4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid	-	?
poly(beta-(1,4)-D-mannuronate)	-	Thalassotalea crassostreae	4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + ?	-	?
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)	-	Thalassotalea crassostreae	4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 80000, SDS-PAGE	Thalassotalea crassostreae

Synonyms

Synonyms	Comment	Organism
ALG1	-	Thalassotalea crassostreae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Thalassotalea crassostreae

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	30	about 85% of maximum activity	Thalassotalea crassostreae
40	-	rapid loss of activity above	Thalassotalea crassostreae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25	-	poly(beta-(1,4)-D-mannuronate)	pH 7.0, 30°C	Thalassotalea crassostreae
41.7	-	poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)	pH 7.0, 30°C	Thalassotalea crassostreae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Thalassotalea crassostreae

pH Range

pH Minimum	pH Maximum	Comment	Organism
2	-	20% residual activity	Thalassotalea crassostreae

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Release 2023.1 (January 2023)