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Literature summary for 4.2.2.B6 extracted from
- on the mechanism of peptidoglycan binding and cleavage by the endo-specific lytic transglycosylase MltE from Escherichia coli (2012), Biochemistry, 51, 9164-9177.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzyme E64Q is expressed in Escherichi coli strain B834(DE3) pLysS | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with the glycopeptide inhibitor bulgecin A and N-acetylglucosaminyl-N-acetylmuramyl-L-Ala-D-Glu, hanging drop vapor diffusion method, using 0.1 M lithium sulfate, 15% (w/v) PEG 8000 in 20 mM HEPES buffer, pH 7.5 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E64Q | inactive | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | a periplasmic, outer membrane-attached enzyme | Escherichia coli | 16020 | - |
| periplasm | - |
Escherichia coli | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0C960 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Micrococcus luteus peptidoglycan | - |
Escherichia coli | ? | - |
? |  |
| additional information | the enzyme cleaves the beta-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in the cell wall peptidoglycan, producing 1,6-anhydromuropeptides | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | in solution, the enzyme behaves as a monomer, as judged from size exclusion chromatography and dynamic light scattering | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endolytic peptidoglycan lytic transglycosylase | - |
Escherichia coli |
| GlcNAc-beta-(1->4)-Mur2Acoyl-L-Ala-gamma-D-Glu lyase (1,6-anhydromuramoyl-residue forming) | systematic name | Escherichia coli |
| MltE | - |
Escherichia coli |
| muropeptide lyase | recommended name | Escherichia coli |
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