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Literature summary for 4.2.2.8 extracted from

  • Hyun, Y.J.; Lee, J.H.; Kim, D.H.
    Cloning, overexpression, and characterization of recombinant heparinase III from Bacteroides stercoris HJ-15 (2010), Appl. Microbiol. Biotechnol., 86, 879-890.
    View publication on PubMed
Search for more literature for 4.2.2.8

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3) Bacteroides stercoris

Protein Variants

Protein Variants Comment Organism
C352A 87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) Bacteroides stercoris
C474A 32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) Bacteroides stercoris
C577A 98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) Bacteroides stercoris
C624A 24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) Bacteroides stercoris

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM: 0% activity Bacteroides stercoris
Pb2+ 1 mM: 0% activity Bacteroides stercoris
Zn2+ 1 mM: 0% activity Bacteroides stercoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 heparan sulfate 0.08808 mg/ml Bacteroides stercoris
additional information
-
 heparin 0.42275 mg/ml Bacteroides stercoris

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM: 109% activity Bacteroides stercoris
Ca2+ 1 mM: 110% activity Bacteroides stercoris
additional information optimal condition: 350 mM NaCl Bacteroides stercoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74930
-
 x * 74930, calculated for mature protein Bacteroides stercoris

Organism

Organism UniProt Comment Textmining
Bacteroides stercoris C7EXL6
-
-
Bacteroides stercoris HJ-15 C7EXL6
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Bacteroides stercoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C Bacteroides stercoris
1.7
-
 hyaluronic acid as substrate Bacteroides stercoris
21.46
-
 heparin as substrate Bacteroides stercoris
32.3
-
 heparan sulfate (porcine intestine) as substrate Bacteroides stercoris
51.81
-
 heparan sulfate (bovine kidney) as substrate Bacteroides stercoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparan sulfate
-
 Bacteroides stercoris n alpha-deltaUA-glcNAc major product ?
heparan sulfate
-
 Bacteroides stercoris HJ-15 n alpha-deltaUA-glcNAc major product ?
heparin
-
 Bacteroides stercoris ?
-
 ?
heparin
-
 Bacteroides stercoris HJ-15 ?
-
 ?
hyaluronic acid
-
 Bacteroides stercoris ?
-
 ?
hyaluronic acid
-
 Bacteroides stercoris HJ-15 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74930, calculated for mature protein Bacteroides stercoris

Synonyms

Synonyms Comment Organism
heparinase III
-
 Bacteroides stercoris
heparitinase
-
 Bacteroides stercoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacteroides stercoris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
42 48 activity above 90% Bacteroides stercoris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 about 20% activity after 1 h at 45°C Bacteroides stercoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Bacteroides stercoris

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Bacteroides stercoris

pI Value

Organism Comment pI Value Maximum pI Value
Bacteroides stercoris calculated for mature protein
-
 8.65