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Literature summary for 4.2.2.7 extracted from

  • Banga, J.; Tripathi, C.K.M.
    Purification and characterization of a novel heparin degrading enzyme from Aspergillus flavus (MTCC-8654) (2010), Appl. Biochem. Biotechnol., 160, 1004-1016.
    View publication on PubMed
Search for more literature for 4.2.2.7

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Aspergillus flavus
dithiothreitol
-
 Aspergillus flavus
EDTA
-
 Aspergillus flavus
additional information free sulfohydryl groups of cysteine residues are necessary for catalytic activity of the enzyme Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Aspergillus flavus
Cd2+
-
 Aspergillus flavus
diethyl dicarbonate
-
 Aspergillus flavus
iodoacetic acid
-
 Aspergillus flavus
Pb2+
-
 Aspergillus flavus
Zn2+
-
 Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 heparin pH 7.5, 30°C Aspergillus flavus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Aspergillus flavus 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Aspergillus flavus
Mn2+ activates Aspergillus flavus
NaCl best activating at 300 mM Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23380
-
 x * 24000, SDS-PAGE, x * 23380, mass spectrometry Aspergillus flavus
24000
-
 x * 24000, SDS-PAGE Aspergillus flavus
24000
-
 x * 24000, SDS-PAGE, x * 23380, mass spectrometry Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus flavus affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products ?
-
 ?
additional information Aspergillus flavus MTCC-8654 affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-
Aspergillus flavus
-
 phylogenetic tree
-
Aspergillus flavus MTCC-8654
-
-
-
Aspergillus flavus MTCC-8654
-
 phylogenetic tree
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 40.5fold by anion and cation exchange chromatography, and gel filtration Aspergillus flavus
native enzyme 40.5fold by anion exchange and hydrophobic interaction chromatography, and gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44.6
-
 purified enzyme, pH 7.5, 30°C Aspergillus flavus
44.6
-
 purified enzyme, pH 7.0, 30°C Aspergillus flavus

Storage Stability

Storage Stability Organism
-20°C, purified native enzyme, 72 h, below 12% loss of activity Aspergillus flavus
4°C, purified native enzyme, 72 h, below 22% loss of activity Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin substrate is heparin from porcine intestinal mucosa. Cysteine, histidine may be present at the active site Aspergillus flavus heparin disaccharide + ?
-
 ?
heparin substrate is heparin from porcine intestinal mucosa. Cysteine, histidine may be present at the active site Aspergillus flavus MTCC-8654 heparin disaccharide + ?
-
 ?
heparin main product Aspergillus flavus unsaturated heparin disaccharides
-
 ?
heparin main product Aspergillus flavus MTCC-8654 unsaturated heparin disaccharides
-
 ?
additional information affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products Aspergillus flavus ?
-
 ?
additional information cysteine, histidine may be present at the active site Aspergillus flavus ?
-
 ?
additional information affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products Aspergillus flavus MTCC-8654 ?
-
 ?
additional information cysteine, histidine may be present at the active site Aspergillus flavus MTCC-8654 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Aspergillus flavus
? x * 24000, SDS-PAGE, x * 23380, mass spectrometry Aspergillus flavus

Synonyms

Synonyms Comment Organism
heparin degrading enzyme
-
 Aspergillus flavus
Heparinase
-
 Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus flavus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 60 highly active within the temperature range of 25-35°C, but activity is lost rapidly above 35°C Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28
-
 purified native enzyme, catalytic activity after about 72 h shows a loss of 40.4% in activity Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Aspergillus flavus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 high activity range Aspergillus flavus

Expression

Organism Comment Expression
Aspergillus flavus inducible heparinase up

General Information

General Information Comment Organism
additional information free sulfohydryl groups of cysteine residues are necessary for catalytic activity of the enzyme Aspergillus flavus