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Literature summary for 4.2.2.7 extracted from
- Cloning, expression and characterization of acharan sulfate-degrading heparin lyase II from Bacteroides stercoris HJ-15 (2010), J. Appl. Microbiol., 108, 226-235.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Bacteroides stercoris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Bacteroides stercoris |  |
| Cu2+ | - |
Bacteroides stercoris | |
| Ni2+ | - |
Bacteroides stercoris | |
| Pb2+ | - |
Bacteroides stercoris | |
| Zn2+ | - |
Bacteroides stercoris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
heparan sulfate | 0.0000182 g/ml | Bacteroides stercoris | |
| additional information | - |
heparin | 0.00002095 g/ml | Bacteroides stercoris | |
| additional information | - |
acharan sulfate | 0.00003799 g/ml | Bacteroides stercoris |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Bacteroides stercoris | |
| Ca2+ | - |
Bacteroides stercoris | |
| Mg2+ | - |
Bacteroides stercoris | |
| Mn2+ | - |
Bacteroides stercoris | |
| additional information | optimal condition: 75 mmol/l NaCl | Bacteroides stercoris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85400 | - |
x * 85400, calculated | Bacteroides stercoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides stercoris | C0JBW5 | - |
- |
| Bacteroides stercoris HJ-15 | C0JBW5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography | Bacteroides stercoris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no activity with chondroitin sulfate or hyaluronic acid | Bacteroides stercoris |
| 6.38 | - |
heparin | Bacteroides stercoris |
| 27.74 | - |
heparan sulfate | Bacteroides stercoris |
| 109.9 | - |
acharan sulfate | Bacteroides stercoris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, without any additives, 15 days, retains its activity, more stable at 4°C than at -20°C, activity decreases after a few cycles of freezing and thawing | Bacteroides stercoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acharan sulfate | uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid | Bacteroides stercoris | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
| acharan sulfate | uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid | Bacteroides stercoris HJ-15 | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
| heparan sulfate | - |
Bacteroides stercoris | ? | 5 products found, major product not identified | ? | |
| heparan sulfate | - |
Bacteroides stercoris HJ-15 | ? | 5 products found, major product not identified | ? | |
| heparin | - |
Bacteroides stercoris | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? | 2 minor products found | ? | |
| heparin | - |
Bacteroides stercoris HJ-15 | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? | 2 minor products found | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85400, calculated | Bacteroides stercoris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heparin lyase II | - |
Bacteroides stercoris |
| heparinase II | - |
Bacteroides stercoris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Bacteroides stercoris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | - |
Bacteroides stercoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
substrate heparin | Bacteroides stercoris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Bacteroides stercoris |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacteroides stercoris | calculated for mature protein | - |
7.35 |
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