Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pedobacter heparinus | - |
- |
extracellular | the fungus produces heparinase, both extracellularly and intracellularly, in its late log phase of growth | Aspergillus oryzae | - |
- |
intracellular | - |
Pedobacter heparinus | 5622 | - |
intracellular | the fungus produces heparinase, both extracellularly and intracellularly, in its late log phase of growth | Aspergillus oryzae | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Pedobacter heparinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enhancement of enzyme production by 92% is achieved by manipulation of the fermentation conditions (pH: 6.5, inoculum size of 0.5-1.0%, incubation temperature 30°C) and medium ingredients (replacement of dextrose by mannitol, soy peptone by chitin and ammonium sulfate by ammonium nitrate and supplementation of pyridoxine hydrochloride and glutaric acid) | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | optimization of enzyme production, simple carbon sources, including sucrose, lactose, maltose, and complex carbon sources such as starch and cellulose are able to support growth of the organism, but enzyme can be produced only in the presence of maltose, sorbitol, mannitol, arabinose, and mannose. Phosphate inhibits enzyme production and cell growth, while heparin inhibits cell growth but is required for enzyme induction, overview. Heparinase production in optimized medium is highest, i.e., 75.4 units, when the pH of the medium is kept at pH 6.5, inoculum size of 0.5-1.0% of spore suspension, and for an incubation temperature of 30°C. Growth profile, heparin utilization, and heparinase production in optimized medium, overview | Pedobacter heparinus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
heparinase activity is found to be dependent on the rate of utilization of heparin during growth. Heparin is taken up rapidly in the initial exponential phase of the growth at a rate of 0.16 g/cell/h. Enzyme activity increases at a rate of 1.53 units/h with the exhaustion of heparin in the medium | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin | - |
Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Heparinase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aspergillus oryzae |
Organism | Comment | Expression |
---|---|---|
Pedobacter heparinus | phosphate suppresses the enzyme production in cells, enzyme production is also reduced by potassium nitrate, maltose, mannose, and especially by sucrose, overview | down |
Pedobacter heparinus | heparin induces the enzyme in cells, enzyme production is also upleveled by addition of N-acetyl D-glucosamine, glutaric acid, mannitol, arabinose, pyridoxine, diammonium biphosphate, ammonium nitrate, and especially by chitin, slight enhancement by L-leucine, L-glutamine, and L-histidine, overview | up |