Literature summary for 4.2.2.7 extracted from

Yapeng, C.; Ningguo, G.; Xiulan, C.; Jing, Y.; Shijun, Q.; Shuzheng, Z.
Rapid purification, characterization and substrate specificity of heparinase from a novel species of Sphingobacterium (2003), J. Biochem., 134, 365-371.

Search for more literature for 4.2.2.7

Activating Compound

Activating Compound	Comment	Organism	Structure
Urea	up to 1 M of urea	Sphingobacterium sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
N-Acetylimidazole	80% inhibition at 1 mM concentration of inhibitor, indicates the necessity of tyrosine residues in the active site	Sphingobacterium sp.
N-bromosuccinimide	30% inhibition at 1 mM concentration of inhibitor	Sphingobacterium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.042	-	N-acetyl-de-O-sulfated heparin	pH 7.4, 37ºC	Sphingobacterium sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Sphingobacterium sp.	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70100	-	1 * 70100, SDS-PAGE	Sphingobacterium sp.
75670	-	mass spectrometry	Sphingobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Sphingobacterium sp.	-	novel species isolated from the effluents of a food mill	-

Purification (Commentary)

Purification (Comment)	Organism
three steps osmotic shock procedure, followed by chromatography on SP-Sepharose and Source 30S resin	Sphingobacterium sp.

Storage Stability

Storage Stability	Organism
unstable if stored at 4ºC, stability is increased with bovine serum albumin addition	Sphingobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
de-N-sulfated heparin	8% activity compared to heparin	Sphingobacterium sp.	?	-	?
deaminated heparin	54% activity compared to heparin	Sphingobacterium sp.	?	-	?
heparan sulfate	bovine or porcine	Sphingobacterium sp.	?	-	?
additional information	chondroitin sulfate A,B or C, and hyaluronic acid can not act as substrates	Sphingobacterium sp.	?	-	?
N-acetyl heparin	78% activity compared to heparin	Sphingobacterium sp.	?	-	?
N-acetyl-de-O-sulfated heparin	250% activity compared to heparin	Sphingobacterium sp.	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 70100, SDS-PAGE	Sphingobacterium sp.

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Release 2023.1 (January 2023)