Literature summary for 4.2.2.5 extracted from

Lunin, V.V.; Li, Y.; Linhardt, R.J.; Miyazono, H.; Kyogashima, M.; Kaneko, T.; Bell, A.W.; Cygler, M.
High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism (2004), J. Mol. Biol., 337, 367-386.

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme alone and in complex with substrates chondroitin 4-sulfate tetrasaccharide, and hyaluronan tetrasaccharide, hanging drop vapour diffusion method, 0.002 ml protein solution: 10 mg/ml protein, + 0.002 ml reservoir solution: 23% w/v PEG 8000, 0.1 M sodium phosphate, pH 6.4, 0.4 M ammonium acetate, 10% v/v glycerol, suspended over 1 ml reservoir solution, a few days, larger crystals by macroseeding, X-ray diffraction structure determination and analysis of the enzyme-substrate complexes at 1.25-1.9 A high resolution	Paenarthrobacter aurescens

Crystallization (Comment)

Organism

native enzyme alone and in complex with substrates chondroitin 4-sulfate tetrasaccharide, and hyaluronan tetrasaccharide, hanging drop vapour diffusion method, 0.002 ml protein solution: 10 mg/ml protein, + 0.002 ml reservoir solution: 23% w/v PEG 8000, 0.1 M sodium phosphate, pH 6.4, 0.4 M ammonium acetate, 10% v/v glycerol, suspended over 1 ml reservoir solution, a few days, larger crystals by macroseeding, X-ray diffraction structure determination and analysis of the enzyme-substrate complexes at 1.25-1.9 A high resolution

Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79500	79840	mass spectrometry	Paenarthrobacter aurescens

Organism

Organism	UniProt	Comment	Textmining
Paenarthrobacter aurescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Paenarthrobacter aurescens

Reaction

Reaction	Comment	Organism	Reaction ID
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]	acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate, lytic mechanism with Tyr242 acting as a general base that abstracts the proton from the C5 position of glucuronic acid while Asn183 and His233 neutralize the charge on the glucuronate acidic group, substrate binding site	Paenarthrobacter aurescens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
chondroitin sulfate A	i.e. chondroitin 4-sulfate	Paenarthrobacter aurescens	?	-	?
chondroitin sulfate C	i.e. chondroitin 6-sulfate	Paenarthrobacter aurescens	?	-	?
hyaluronan	-	Paenarthrobacter aurescens	?	-	?
additional information	substrate specificity, no activity with dermatan sulfate	Paenarthrobacter aurescens	?	-	?

Synonyms

Synonyms	Comment	Organism
chondroitin lyase AC	-	Paenarthrobacter aurescens