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Literature summary for 4.2.2.5 extracted from

  • Capila, I.; Wu, Y.; Rethwisch, D.W.; Matte, A.; Cygler, M.; Linhardt, R.J.
    Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum (2002), Biochim. Biophys. Acta, 1597, 260-270.
    View publication on PubMed
Search for more literature for 4.2.2.5

Cloned(Commentary)

Cloned (Comment) Organism
mutant R292A, expression in Escherichia coli and conjugation to Flavobacterium heparinum to create a mutant strain Pedobacter heparinus

Protein Variants

Protein Variants Comment Organism
R292A site-directed mutagenesis, substrate recognition residue, reduced activity, action pattern is neither purely random endolytic nor purely random exolytic Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00286
-
 hyaluronic acid wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
0.00528
-
 chondroitin 6-sulfate wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
0.00544
-
 chondroitin 4-sulfate wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
0.0231
-
 hyaluronic acid mutant R292A, pH 8.0, 37°C Pedobacter heparinus
0.0322
-
 chondroitin wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
0.0563
-
 chondroitin 6-sulfate mutant R292A, pH 8.0, 37°C Pedobacter heparinus
0.0651
-
 chondroitin 4-sulfate mutant R292A, pH 8.0, 37°C Pedobacter heparinus
0.119
-
 chondroitin mutant R292A, pH 8.0, 37°C Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant from mutant Flavobacterium heparinum strain Pedobacter heparinus

Reaction

Reaction Comment Organism Reaction ID
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate, reaction mechanism Pedobacter heparinus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin best substrate Pedobacter heparinus ?
-
 ?
chondroitin sulfate A i.e. chondroitin 4-sulfate Pedobacter heparinus ?
-
 ?
chondroitin sulfate C i.e. chondroitin 6-sulfate Pedobacter heparinus ?
-
 ?
hyaluronic acid
-
 Pedobacter heparinus ?
-
 ?
additional information the active site forms a tunnel, structurally involved residue Arg292 is responsible for substrate recognition of N-acetyl or O-sulfo moieties in galactosamine residues, it does not participate in catalysis Pedobacter heparinus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pedobacter heparinus