Cloned (Comment) | Organism |
---|---|
gene cAC, DNA sequence determination and analysis, functional expression without the N-terminal signal peptide in Escherichia coli as His-tagged protein, 2 different expression systems | Pedobacter heparinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | 0.8 | chondroitin 6-sulfate | recombinant enzyme, pH 8.0, 35°C | Pedobacter heparinus | |
0.6 | 0.8 | chondroitin 4-sulfate | recombinant enzyme, pH 8.0, 35°C | Pedobacter heparinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78030 | - |
mass spectrometry | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | - |
gene cAC | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme contains a N-terminal signal peptide which is proteolytically cleaved to produce an active enzyme | Pedobacter heparinus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged active enzyme from Escherichia coli, 66fold | Pedobacter heparinus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] | acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate | Pedobacter heparinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
541 | - |
purified recombinant enzyme | Pedobacter heparinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin sulfate A | i.e. chondroitin 4-sulfate | Pedobacter heparinus | unsaturated chondroitin A sulfate disaccharide | - |
? | |
chondroitin sulfate C | i.e. chondroitin 6-sulfate | Pedobacter heparinus | unsaturated chondroitin sulfate C disaccharide | product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
chondroitinase AC | - |
Pedobacter heparinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Pedobacter heparinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
234 | - |
chondroitin 4-sulfate | recombinant enzyme, pH 8.0, 35°C, dependent on expression system | Pedobacter heparinus | |
423 | 480 | chondroitin 6-sulfate | recombinant enzyme, pH 8.0, 35°C, dependent on expression system | Pedobacter heparinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pedobacter heparinus |