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Literature summary for 4.2.2.5 extracted from

  • Pojasek, K.; Shriver, Z.; Kiley, P.; Venkataraman, G.; Sasisekharan, R.
    Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum (2001), Biochem. Biophys. Res. Commun., 286, 343-351.
    View publication on PubMed
Search for more literature for 4.2.2.5

Cloned(Commentary)

Cloned (Comment) Organism
gene cAC, DNA sequence determination and analysis, functional expression without the N-terminal signal peptide in Escherichia coli as His-tagged protein, 2 different expression systems Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6 0.8 chondroitin 6-sulfate recombinant enzyme, pH 8.0, 35°C Pedobacter heparinus
0.6 0.8 chondroitin 4-sulfate recombinant enzyme, pH 8.0, 35°C Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78030
-
 mass spectrometry Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
 gene cAC
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme contains a N-terminal signal peptide which is proteolytically cleaved to produce an active enzyme Pedobacter heparinus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged active enzyme from Escherichia coli, 66fold Pedobacter heparinus

Reaction

Reaction Comment Organism Reaction ID
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate Pedobacter heparinus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
541
-
 purified recombinant enzyme Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A i.e. chondroitin 4-sulfate Pedobacter heparinus unsaturated chondroitin A sulfate disaccharide
-
 ?
chondroitin sulfate C i.e. chondroitin 6-sulfate Pedobacter heparinus unsaturated chondroitin sulfate C disaccharide product analysis ?

Synonyms

Synonyms Comment Organism
chondroitinase AC
-
 Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Pedobacter heparinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
234
-
 chondroitin 4-sulfate recombinant enzyme, pH 8.0, 35°C, dependent on expression system Pedobacter heparinus
423 480 chondroitin 6-sulfate recombinant enzyme, pH 8.0, 35°C, dependent on expression system Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pedobacter heparinus