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Literature summary for 4.2.2.5 extracted from

  • Rye, C.S.; Withers, S.G.
    Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates (2002), Anal. Biochem., 308, 77-82.
    View publication on PubMed
Search for more literature for 4.2.2.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 phenyl 4-methylumbelliferyl-beta-D-glucopyranosiduronic acid pH 8.0, 30°C Pedobacter heparinus
7
-
 benzyl 4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiduronic acid pH 6.8, 30°C Pedobacter heparinus
28
-
 benzyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid pH 6.8, 30°C Pedobacter heparinus
114
-
 phenyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid pH 6.8, 30°C Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate, random endolytic elimination mechanism Pedobacter heparinus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid synthetic chromogenic substrate Pedobacter heparinus ?
-
 ?
benzyl 4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiduronic acid synthetic chromogenic substrate Pedobacter heparinus ?
-
 ?
chondroitin sulfate A i.e. chondroitin 4-sulfate Pedobacter heparinus ?
-
 ?
chondroitin sulfate C i.e. chondroitin 6-sulfate Pedobacter heparinus ?
-
 ?
additional information developement of an assay using synthetic substrates, which can be monitored by 3 different techniques, UV-Vis spectroscopy, fluorescence spectroccopy, and fluoride ion-selective electrode usage, overview, products of chondroitin sulfate cleavages are disaccharides or oligosaccharides with DELTA4,5-unsaturated uronic acid resdiues at the nonreducing end, methyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid is a poor substrate Pedobacter heparinus ?
-
 ?
phenyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid synthetic chromogenic substrate Pedobacter heparinus ?
-
 ?
phenyl 4-methylumbelliferyl-beta-D-glucopyranosiduronic acid synthetic chromogenic substrate Pedobacter heparinus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 phenyl 4-methylumbelliferyl-beta-D-glucopyranosiduronic acid pH 8.0 Pedobacter heparinus
0.019
-
 benzyl 4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiduronic acid pH 6.8 Pedobacter heparinus
0.16
-
 benzyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid pH 6.8 Pedobacter heparinus
2.3
-
 phenyl 4-deoxy-4-fluoro-beta-D-glucopyranosiduronic acid pH 6.8 Pedobacter heparinus