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Literature summary for 4.2.2.5 extracted from

  • Hiyama, K.; Okada, S.
    Multiple forms of chondroitinase AC from Arthrobacter aurescens (1977), Agric. Biol. Chem., 41, 1279-1286.
No PubMed abstract available
Search for more literature for 4.2.2.5

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, chondroitinase I, II and III, SDS-PAGE Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens
-
 multiple forms: chondroitinase I, chondroitinase II and chondroitinase III
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification carbohydrate content of chondroitinase I is 4.1%, carbohydrate content of chondroitinase II is 2.7%, carbohydrate content of chondroitinase III is 3.1% Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin degradation is faster than with chondroitin 4-sulfate or chondroitin 6-sulfate Paenarthrobacter aurescens ?
-
 ?
chondroitin 4-sulfate
-
 Paenarthrobacter aurescens 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose
-
 ?
chondroitin 6-sulfate
-
 Paenarthrobacter aurescens ?
-
 ?
hyaluronate degradation is faster than with chondroitin 4-sulfate or chondroitin 6-sulfate Paenarthrobacter aurescens ?
-
 ?
additional information no activity with dermatan sulfate Paenarthrobacter aurescens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, chondroitinase I, II and III, SDS-PAGE Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenarthrobacter aurescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
-
 Paenarthrobacter aurescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 6.0, 15 min, stable below 45°C Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Paenarthrobacter aurescens

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5: about 25% of maximal activity, pH 8: about 35% of maximal activity Paenarthrobacter aurescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 40°C, 2 h, no loss of activity Paenarthrobacter aurescens