Crystallization (Comment) | Organism |
---|---|
crystal structure at 1.77 A resolution and putative substrate-binding model. The enzyme adopts a beta-jelly roll fold at the core of the structure and residues Lys99, Tyr140, and Tyr142 form catalytic residues in the active site | Aplysia kurodai |
Protein Variants | Comment | Organism |
---|---|---|
C115A/C124G | strong loss of activity | Aplysia kurodai |
G118N | almost complete loss of activity | Aplysia kurodai |
Y140F | mutation in active site residue, 1.3% of wild-type activity | Aplysia kurodai |
Y142F | mutation in active site residue, 0.6% of wild-type activity | Aplysia kurodai |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
poly(beta-(1,4)-D-mannuronate) | Km value 0.79 mg/ml, wild-type, pH 7.0, 30°C | Aplysia kurodai | |
additional information | - |
alginate | Km value 0.95 mg/ml, wild-type, pH 7.0, 30°C | Aplysia kurodai | |
additional information | - |
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) | Km value 68.8 mg/ml, wild-type, pH 7.0, 30°C | Aplysia kurodai |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aplysia kurodai | E7FLQ2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
substrate poly(alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C | Aplysia kurodai |
0.03 | - |
substrate poly(alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C | Aplysia kurodai |
0.08 | - |
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C | Aplysia kurodai |
0.11 | - |
substrate alginate, mutant Y142F, pH 7.0, 30°C | Aplysia kurodai |
0.16 | - |
substrate poly(alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C | Aplysia kurodai |
0.18 | - |
substrate poly(beta-(1,4)-D-mannuronate), mutant G118N, pH 7.0, 30°C | Aplysia kurodai |
0.22 | - |
substrate alginate, mutant Y140F, pH 7.0, 30°C | Aplysia kurodai |
0.31 | - |
substrate alginate, mutant G118N, pH 7.0, 30°C | Aplysia kurodai |
0.83 | - |
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C | Aplysia kurodai |
2.2 | - |
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C | Aplysia kurodai |
6 | - |
substrate alginate, mutant C115A/C124G, pH 7.0, 30°C | Aplysia kurodai |
6.8 | - |
substrate poly(beta-(1,4)-D-mannuronate), mutant C115A/C124G, pH 7.0, 30°C | Aplysia kurodai |
17.4 | - |
substrate alginate, wild-type, pH 7.0, 30°C | Aplysia kurodai |
33 | - |
substrate poly(beta-(1,4)-D-mannuronate), wild-type, pH 7.0, 30°C | Aplysia kurodai |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Aplysia kurodai | unsaturated algino-oligosaccharides | - |
? | |
poly(alpha-(1,4)-L-guluronate) | reaction of EC 4.2.2.11 | Aplysia kurodai | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
poly(beta-(1,4)-D-mannuronate) | - |
Aplysia kurodai | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) | - |
Aplysia kurodai | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aly30 | - |
Aplysia kurodai |