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Literature summary for 4.2.2.3 extracted from

  • Qin, H.M.; Miyakawa, T.; Inoue, A.; Nishiyama, R.; Nakamura, A.; Asano, A.; Sawano, Y.; Ojima, T.; Tanokura, M.
    Structure and polymannuronate specificity of a eukaryotic member of polysaccharide lyase family 14 (2017), J. Biol. Chem., 292, 2182-2190 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure at 1.77 A resolution and putative substrate-binding model. The enzyme adopts a beta-jelly roll fold at the core of the structure and residues Lys99, Tyr140, and Tyr142 form catalytic residues in the active site Aplysia kurodai

Protein Variants

Protein Variants Comment Organism
C115A/C124G strong loss of activity Aplysia kurodai
G118N almost complete loss of activity Aplysia kurodai
Y140F mutation in active site residue, 1.3% of wild-type activity Aplysia kurodai
Y142F mutation in active site residue, 0.6% of wild-type activity Aplysia kurodai

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(beta-(1,4)-D-mannuronate) Km value 0.79 mg/ml, wild-type, pH 7.0, 30°C Aplysia kurodai
additional information
-
 alginate Km value 0.95 mg/ml, wild-type, pH 7.0, 30°C Aplysia kurodai
additional information
-
 poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) Km value 68.8 mg/ml, wild-type, pH 7.0, 30°C Aplysia kurodai

Organism

Organism UniProt Comment Textmining
Aplysia kurodai E7FLQ2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 substrate poly(alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C Aplysia kurodai
0.03
-
 substrate poly(alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C Aplysia kurodai
0.08
-
 substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C Aplysia kurodai
0.11
-
 substrate alginate, mutant Y142F, pH 7.0, 30°C Aplysia kurodai
0.16
-
 substrate poly(alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C Aplysia kurodai
0.18
-
 substrate poly(beta-(1,4)-D-mannuronate), mutant G118N, pH 7.0, 30°C Aplysia kurodai
0.22
-
 substrate alginate, mutant Y140F, pH 7.0, 30°C Aplysia kurodai
0.31
-
 substrate alginate, mutant G118N, pH 7.0, 30°C Aplysia kurodai
0.83
-
 substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C Aplysia kurodai
2.2
-
 substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C Aplysia kurodai
6
-
 substrate alginate, mutant C115A/C124G, pH 7.0, 30°C Aplysia kurodai
6.8
-
 substrate poly(beta-(1,4)-D-mannuronate), mutant C115A/C124G, pH 7.0, 30°C Aplysia kurodai
17.4
-
 substrate alginate, wild-type, pH 7.0, 30°C Aplysia kurodai
33
-
 substrate poly(beta-(1,4)-D-mannuronate), wild-type, pH 7.0, 30°C Aplysia kurodai

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Aplysia kurodai unsaturated algino-oligosaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11 Aplysia kurodai 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Aplysia kurodai 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)
-
 Aplysia kurodai 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?

Synonyms

Synonyms Comment Organism
Aly30
-
 Aplysia kurodai