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Literature summary for 4.2.2.3 extracted from

  • Inoue, A.; Anraku, M.; Nakagawa, S.; Ojima, T.
    Discovery of a novel alginate lyase from Nitratiruptor sp. SB155-2 thriving at deep-sea hydrothermal vents and identification of the residues responsible for its heat stability (2016), J. Biol. Chem., 291, 15551-15563 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nitratiruptor sp. SB155-2

Protein Variants

Protein Variants Comment Organism
additional information introduction of two cysteines based on homology modeling using Pseudomonas aeruginosa alginate lyase PA1167 as the template enhances heat stability Nitratiruptor sp. SB155-2

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM, 69% inhibition Nitratiruptor sp. SB155-2
Fe3+ 1 mM, 86% inhibition Nitratiruptor sp. SB155-2
Pb2+ 1 mM, 82% inhibition Nitratiruptor sp. SB155-2
Sn2+ 1 mM, 85% inhibition Nitratiruptor sp. SB155-2

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 0.8-1.4 M NaCl required for maximum activity Nitratiruptor sp. SB155-2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 PAGE Nitratiruptor sp. SB155-2

Organism

Organism UniProt Comment Textmining
Nitratiruptor sp. SB155-2 A6Q1E0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1620
-
 substrate poly(beta-1,4-D-mannuronate), pH 6.0, 70°C Nitratiruptor sp. SB155-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate about 30% of the activity with poly(beta-1,4-D-mannuronate) Nitratiruptor sp. SB155-2 unsaturated algino-oligosaccharides
-
 ?
poly(alpha-1,4-L-guluronate) about 5% of the activity with poly(beta-1,4-D-mannuronate), reaction of EC 4.2.2.11 Nitratiruptor sp. SB155-2 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?
poly(beta-1,4-D-mannuronate) prefered substrate Nitratiruptor sp. SB155-2 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate)
-
 ?
poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate) about 30% of the activity with poly(beta-1,4-D-mannuronate) Nitratiruptor sp. SB155-2 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26692, calculated from sequence, 1 * 29000, SDS-PAGE Nitratiruptor sp. SB155-2

Synonyms

Synonyms Comment Organism
NIS_0185
-
 Nitratiruptor sp. SB155-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Nitratiruptor sp. SB155-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 heat stability is decreased in the presence of 5 mM dithiotreitol, and residues Cys80 and Cys232 are responsible for heat stability but not lyase activity Nitratiruptor sp. SB155-2
67
-
 30 min, 50% loss of activity Nitratiruptor sp. SB155-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Nitratiruptor sp. SB155-2

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 more than 80% of maximum activity Nitratiruptor sp. SB155-2