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Literature summary for 4.2.2.3 extracted from

  • Peng, C.; Wang, Q.; Lu, D.; Han, W.; Li, F.
    A novel bifunctional endolytic alginate lyase with variable alginate-degrading modes and versatile monosaccharide-producing properties (2018), Front. Microbiol., 9, 167 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flammeovirga sp. MY04

Protein Variants

Protein Variants Comment Organism
T282N deletion of the noncatalytic region, 1.5fold increase in specific activity compared to wild-type Flammeovirga sp. MY04

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 alginate Km value of wild-type 0.135 mg/ml, of mutant T282N 0.158 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04
additional information
-
 poly(alpha-(1,4)-L-guluronate) Km value of wild-type 0.706 mg/ml, of mutant T282N 1.224 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04
additional information
-
 poly(beta-(1,4)-D-mannuronate) Km value of wild-type 2.845 mg/ml, of mutant T282N 0.392 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04

Organism

Organism UniProt Comment Textmining
Flammeovirga sp. MY04 A0A1B1FUS6
-
-

Purification (Commentary)

Purification (Comment) Organism
purification using Ni-NTA column Flammeovirga sp. MY04

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2025
-
 wild-type, pH 6.0, 40°C Flammeovirga sp. MY04
2920
-
 mutant T282N, pH 6.0, 40°C Flammeovirga sp. MY04

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11, about 15% of the activity with poly(beta-(1,4)-D-mannuronate) Flammeovirga sp. MY04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Flammeovirga sp. MY04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 60580, calculated from sequence Flammeovirga sp. MY04

Synonyms

Synonyms Comment Organism
Aly2
-
 Flammeovirga sp. MY04
MY04_2544
-
 Flammeovirga sp. MY04

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 wild-type, 2 h, less than 30% residual activity. Mutant T282N, 8 h, 50% residual activity Flammeovirga sp. MY04

pI Value

Organism Comment pI Value Maximum pI Value
Flammeovirga sp. MY04 calculated from sequence
-
 9.1