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Literature summary for 4.2.2.3 extracted from

  • Yagi, H.; Fujise, A.; Itabashi, N.; Ohshiro, T.
    Purification and characterization of a novel alginate lyase from the marine bacterium Cobetia sp. NAP1 isolated from brown algae (2016), Biosci. Biotechnol. Biochem., 80, 2338-2346 .
    View publication on PubMed
Search for more literature for 4.2.2.3

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong inhibition Cobetia sp. NAP1

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Cobetia sp. NAP1
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimum activity at 0.25 M. At 2 M, about 70% residual activity Cobetia sp. NAP1
Na+ optimum activity at 0.25 M. At 2 M, about 70% residual activity Cobetia sp. NAP1

Organism

Organism UniProt Comment Textmining
Cobetia sp. NAP1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide Cobetia sp. NAP1

Purification (Commentary)

Purification (Comment) Organism
-
 Cobetia sp. NAP1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Cobetia sp. NAP1 algino-monosaccharides + algino-disaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11 Cobetia sp. NAP1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Cobetia sp. NAP1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)
-
 Cobetia sp. NAP1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, x * 35400, MALDI-TOF, x * 35700, calculated from sequence, mature protein Cobetia sp. NAP1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cobetia sp. NAP1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, about 80% residual activity Cobetia sp. NAP1
80
-
 15 min, about 30% residual activity Cobetia sp. NAP1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cobetia sp. NAP1

pH Range

pH Minimum pH Maximum Comment Organism
6 9 at least 50% of maximum activity Cobetia sp. NAP1

pI Value

Organism Comment pI Value Maximum pI Value
Cobetia sp. NAP1 calculated from sequence, mature protein
-
 5