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Literature summary for 4.2.2.3 extracted from

  • Cheng, Y.; Wang, D.; Gu, J.; Li, J.; Liu, H.; Li, F.; Han, W.
    Biochemical characteristics and variable alginate-degrading modes of a novel bifunctional endolytic alginate lyase (2017), Appl. Environ. Microbiol., 83, e01608 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Protein Variants

Protein Variants Comment Organism
T185N NCR-truncated protein, mutant has lost its M-producing capability but retained the ability to yield L-guluronate units by almost completely degrading 2-aminobenzamide-labeled tetra(L-guluronate) chains. Mutant T185N can degrade 2-aminobenzamide-labeled penta(L-guluronate) and 2-aminobenzamide-labeled penta(D-mannuronate) fractions at greater proportions than wild-type Flammeovirga sp. MY04

Organism

Organism UniProt Comment Textmining
Flammeovirga sp. MY04 A0A1B1FUW4 bifunctional alginate lyase, catalyzes the reactions of EC 4.2.2.3 and EC 4.2.2.11
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
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 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
additional information Aly1 is a bifunctional alginate lyase and prefers L-guluronate to D-mannuronate. Tetrasaccharide-size fractions are the smallest substrates, and D-mannuronate, L-guluronate, and UDP2 fractions are the minimal product types. Products are a series of small size-defined saturated oligosaccharide products from the nonreducing ends of single or different saturated sugar chains and yielding unsaturated products in distinct but restricted pattern. No substrates: chondroitin, chondroitin sulfate, dermantan sulfate B, hyaluronan, heparin, or heparin sulfate Flammeovirga sp. MY04 ?
-
 ?
poly(alpha-L-(1,4)-guluronate) reaction of EC 4.2.2.11 Flammeovirga sp. MY04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-D-mannuronate)
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 Flammeovirga sp. MY04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 50650, calculated from sequence Flammeovirga sp. MY04

Synonyms

Synonyms Comment Organism
Aly1
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 Flammeovirga sp. MY04

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Flammeovirga sp. MY04

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 24 h, at least 60% residual activity Flammeovirga sp. MY04

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Flammeovirga sp. MY04

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 2 h, at least 80% residual activity Flammeovirga sp. MY04

pI Value

Organism Comment pI Value Maximum pI Value
Flammeovirga sp. MY04 calculated from sequence
-
 4.2

General Information

General Information Comment Organism
physiological function deletion of the noncatalytic region of Aly1 causes weak changes of biochemical characteristics but increases the degradation proportions of small size-defined saturated D-mannuronate-enriched oligosaccharide substrates and unsaturated tetrasaccharide fractions without any size changes of degradable oligosaccharides Flammeovirga sp. MY04