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Literature summary for 4.2.2.3 extracted from
- Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic (2015), Appl. Environ. Microbiol., 81, 1865-1873 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio splendidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, about 20% of initial activity | Vibrio splendidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
alginate | pH 7.5, 25°C | Vibrio splendidus | |
| 0.036 | - |
alginate | pH 8.5, 25°C | Vibrio splendidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 1.2fold increase in enzymatic activity | Vibrio splendidus | |
| Ca2+ | 1 mM, 1.5fold increase in enzymatic activity | Vibrio splendidus | |
| Na+ | up to 10fold increase in activity, optimum concentration 1 M | Vibrio splendidus | |
| Na+ | up to 3fold increase in activity, optimum concentration 400 mM | Vibrio splendidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
gel filtration | Vibrio splendidus |
| 68200 | - |
gel filtration | Vibrio splendidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio splendidus | A3UR40 | - |
- |
| Vibrio splendidus | A3UR41 | - |
- |
| Vibrio splendidus 12B01 | A3UR40 | - |
- |
| Vibrio splendidus 12B01 | A3UR41 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Vibrio splendidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Vibrio splendidus | unsaturated algino-oligosaccharides | - |
? | |
| alginate | - |
Vibrio splendidus 12B01 | unsaturated algino-oligosaccharides | - |
? | |
| additional information | isoforms AlyA and AlyB principally cleave the beta-1,4 bonds between beta-D-mannuronate and alpha-L-guluronate and subunits | Vibrio splendidus | ? | - |
? | |
| additional information | isoforms AlyD and AlyE principally cleave the alpha-1,4 bonds involving alpha-L-guluronate subunits | Vibrio splendidus | ? | - |
? | |
| additional information | isoforms AlyA and AlyB principally cleave the beta-1,4 bonds between beta-D-mannuronate and alpha-L-guluronate and subunits | Vibrio splendidus 12B01 | ? | - |
? | |
| additional information | isoforms AlyD and AlyE principally cleave the alpha-1,4 bonds involving alpha-L-guluronate subunits | Vibrio splendidus 12B01 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 57500, calculated from sequence, 1 * 62000, SDS-PAGE | Vibrio splendidus |
| monomer | 1 * 67400, calculated from sequence, 1 * 70000, SDS-PAGE | Vibrio splendidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlyA | - |
Vibrio splendidus |
| AlyB | - |
Vibrio splendidus |
| V12B01_24254 | - |
Vibrio splendidus |
| V12B01_24259 | - |
Vibrio splendidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | - |
Vibrio splendidus |
| 25 | - |
- |
Vibrio splendidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
alginate | pH 8.5, 25°C | Vibrio splendidus | |
| 3.7 | - |
alginate | pH 7.5, 25°C | Vibrio splendidus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vibrio splendidus |
| 8.5 | - |
- |
Vibrio splendidus |
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Release 2023.1 (January 2023)
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