Literature summary for 4.2.2.3 extracted from

Sim, S.J.; Baik, K.S.; Park, S.C.; Choe, H.N.; Seong, C.N.; Shin, T.S.; Woo, H.C.; Cho, J.Y.; Kim, D.
Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone (2012), J. Ind. Microbiol. Biotechnol., 39, 585-593.

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Activating Compound

Activating Compound	Comment	Organism	Structure
AgNO3	activates 31% at 1 mM	Klebsiella pneumoniae
CoCl2	activates 8% at 1 mM	Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, lambda phage display, sequence comparisons	Klebsiella pneumoniae

General Stability

General Stability	Organism
AlyDW11 contains three highly conserved regions, RSEL, QIH, and YFKAGVYNQ, which may act to stabilize the three-dimensional conformation and function of the alginate lyase	Klebsiella pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	25% inhibition at 1 mM	Klebsiella pneumoniae
FeSO4	10% inhibition at 1 mM	Klebsiella pneumoniae
KCl	weak inhibition at 1 mM	Klebsiella pneumoniae
MgCl2	weak inhibition at 1 mM	Klebsiella pneumoniae
MgSO4	weak inhibition at 1 mM	Klebsiella pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Klebsiella pneumoniae	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no or poor effects by CaCl2 and NaCl at 1 mM	Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sodium alginate	Klebsiella pneumoniae	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	Q59478	subsp. aerogenes, isolated from the gut of abalone from the South Sea near Yeosu in the Republic of Korea	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly(beta-D-manuronate)	AlyDW11 prefers poly(beta-D-mannuronate) as a substrate over poly(alpha-L-guluronate)	Klebsiella pneumoniae	?	-	?
sodium alginate	-	Klebsiella pneumoniae	?	-	?
sodium alginate	profiles of alginate oligomers are obtained by digestion of alginate with an alginate lyase AlyDW11, overview. The enzyme has endolytic activity and prefers poly(beta-D-mannuronate) over poly(alpha-L-guluronate)	Klebsiella pneumoniae	?	-	?

Synonyms

Synonyms	Comment	Organism
alginate lyase	-	Klebsiella pneumoniae
AlyDW11	-	Klebsiella pneumoniae
M block-specific polymannuronate lyase	-	Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)