Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Isoptericola halotolerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Isoptericola halotolerans | |
Mg2+ | activates | Isoptericola halotolerans | |
Mn2+ | activates | Isoptericola halotolerans | |
Na+ | activates | Isoptericola halotolerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
- |
Isoptericola halotolerans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | Isoptericola halotolerans | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 | ? | - |
? | |
alginate | Isoptericola halotolerans CGMCC5336 | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Isoptericola halotolerans | AB489222 | isolated from rotten seaweed | - |
Isoptericola halotolerans CGMCC5336 | AB489222 | isolated from rotten seaweed | - |
Purification (Comment) | Organism |
---|---|
native enzyme 1.75fold by ammonium sulfate fractionation and anion exchange chromatography | Isoptericola halotolerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8409 | - |
purified native enzyme, pH 7.0, 50°C | Isoptericola halotolerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 | Isoptericola halotolerans | ? | - |
? | |
alginate | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 | Isoptericola halotolerans CGMCC5336 | ? | - |
? | |
poly(beta-D-mannuronate) | - |
Isoptericola halotolerans | ? | - |
? | |
poly(beta-D-mannuronate) | - |
Isoptericola halotolerans CGMCC5336 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000 | Isoptericola halotolerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Isoptericola halotolerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity range, profile overview | Isoptericola halotolerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, loss of 40% activity after 180 min | Isoptericola halotolerans |
50 | - |
purified enzyme, inactivation after 120 min | Isoptericola halotolerans |
60 | - |
purified enzyme, inactivation after 90 min | Isoptericola halotolerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Isoptericola halotolerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | activity range, profile overview | Isoptericola halotolerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | purified enzyme, 12 h, over 60% activity within this pH range remains pH 5.5 to 8.5, mostly stable at pH 7.5, half inactivation at pH 9.5 | Isoptericola halotolerans |