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Literature summary for 4.2.2.3 extracted from

  • Kim, H.T.; Ko, H.J.; Kim, N.; Kim, D.; Lee, D.; Choi, I.G.; Woo, H.C.; Kim, M.D.; Kim, K.H.
    Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans (2012), Biotechnol. Lett., 34, 1087-1092.
    View publication on PubMed
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
gene alg7D, overexpression of soluble His-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21(DE3) Saccharophagus degradans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Saccharophagus degradans
Cu2+
-
 Saccharophagus degradans
Fe2+
-
 Saccharophagus degradans
Mg2+
-
 Saccharophagus degradans
Mn2+
-
 Saccharophagus degradans
Zn2+
-
 Saccharophagus degradans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Saccharophagus degradans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect on activity by K+ Saccharophagus degradans
Na+ slight activation Saccharophagus degradans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32700
-
 x * 32700, recombinant His-tagged enzyme without, SDS-PAGE Saccharophagus degradans

Organism

Organism UniProt Comment Textmining
Saccharophagus degradans
-
 gene alg7D
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme lacking the signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Saccharophagus degradans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.98
-
 wild-type enzyme, pH 7.0, 50°C, substrate alginate Saccharophagus degradans
41.4
-
 purified recombinant His-tagged enzyme, pH 7.0, 50°C, substrate alginate Saccharophagus degradans
67
-
 purified recombinant His-tagged enzyme, substrate poly(beta-D-mannuronate), pH 7.0, 50°C Saccharophagus degradans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate the enzyme endolytically depolymerizes alginate by beta-elimination into oligo-alginates with degrees of polymerization of 2-5 Saccharophagus degradans ?
-
 ?
additional information the enzyme is active on poly(beta-D-mannuronate) and poly(alpha-L-guluronate), cf. EC 4.2.2.11 Saccharophagus degradans ?
-
 ?
poly(beta-D-mannuronate)
-
 Saccharophagus degradans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32700, recombinant His-tagged enzyme without, SDS-PAGE Saccharophagus degradans

Synonyms

Synonyms Comment Organism
Alg7D
-
 Saccharophagus degradans
alginate lyase
-
 Saccharophagus degradans
endo-type alginate lyase
-
 Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Saccharophagus degradans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, profile overview Saccharophagus degradans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Saccharophagus degradans

pH Range

pH Minimum pH Maximum Comment Organism
5 11 activity range, profile overview Saccharophagus degradans

General Information

General Information Comment Organism
evolution the enzyme belongs to the to the polysaccharide lyase-7 family Saccharophagus degradans