Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | A7LFP7 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-Sepharose affinity chromatography | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | enzyme lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products are determined to be di-, tri-, tetra- and pentasaccharides | Streptomyces sp. | unsaturated algino-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALG-5 | - |
Streptomyces sp. |
alginate lyase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces sp. |