Cloned (Comment) | Organism |
---|---|
overexpression of AlgL in Escherichia coli | Pseudomonas aeruginosa |
protein PA1167, overexpression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method, crystal structure determined at 2.0 A resolution. PA1167 forms a glove-like beta-sandwich composed of 15 beta-strands and 3 alpha-helices | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
protein PA1167, gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | Pseudomonas aeruginosa | AlgL plays a main role in alginate depolymerization | unsaturated algino-oligosaccharides | - |
? | |
alginate | Pseudomonas aeruginosa AlgL | AlgL plays a main role in alginate depolymerization | unsaturated algino-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
AlgL | - |
Pseudomonas aeruginosa | - |
protein PA1167 | - |
Pseudomonas aeruginosa AlgL | - |
AlgL | - |
Purification (Comment) | Organism |
---|---|
recombinant AlgL | Pseudomonas aeruginosa |
recombinant protein PA1167 | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0274 | - |
protein PA1167 | Pseudomonas aeruginosa |
0.926 | - |
AlgL | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Pseudomonas aeruginosa | unsaturated algino-oligosaccharides | - |
? | |
alginate | AlgL plays a main role in alginate depolymerization | Pseudomonas aeruginosa | unsaturated algino-oligosaccharides | - |
? | |
alginate | protein PA1167 acts preferably on heteropolymeric regions endolytically | Pseudomonas aeruginosa | unsaturated algino-oligosaccharides | - |
? | |
alginate | - |
Pseudomonas aeruginosa AlgL | unsaturated algino-oligosaccharides | - |
? | |
alginate | AlgL plays a main role in alginate depolymerization | Pseudomonas aeruginosa AlgL | unsaturated algino-oligosaccharides | - |
? | |
alginate | protein PA1167 acts preferably on heteropolymeric regions endolytically | Pseudomonas aeruginosa AlgL | unsaturated algino-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, protein PA1167, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
AlgL | - |
Pseudomonas aeruginosa |
protein PA1167 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
protein PA1167 | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 80% loss of activity, protein PA1167 | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.42 | - |
alginate | protein PA1167, pH 7.5, 37°C | Pseudomonas aeruginosa | |
748 | - |
alginate | AlgL | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
protein PA1167 | Pseudomonas aeruginosa |