Cloned (Comment) | Organism |
---|---|
overexpression of isozyme A1-III in Bacillus subtilis | Sphingomonas sp. |
Crystallization (Comment) | Organism |
---|---|
8 mg/ml purified isozyme A1-III complexed with trisaccharide product 4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid, hanging drop vapour diffusion method, 0.1 M HEPES, pH 7.5, containg 48% PEG w/v saturated ammonium sulfate, 290 mM trisaccharide, 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
isozyme A1-III | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme A1-III from Bacillus subtilis | Sphingomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1 | substrate binding site structure, active site cleft, catalytic mechanism | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-beta-1,4-D-mannuronate | - |
Sphingomonas sp. | 4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Sphingomonas sp. |