Cloned (Comment) | Organism |
---|---|
overexpression of isozyme A1-II in Escherichia coli | Sphingomonas sp. |
Crystallization (Comment) | Organism |
---|---|
purified isozyme A1-II, hanging drop vapour diffusion method, 0.003 ml protein solution: 38 mg/ml protein, 50 mM Tris-HCl, pH 7.5, + 0.003 ml bottom solution: 0.1 M Tris-HCl, pH 8.5, 43% saturated ammonium sulfate, 8% PEG 4000, 0.2 M Li2SO4, 20°C, 1 month, X-ray diffraction structure determination and analysis at 2.8 A resolution | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
isozyme A1-II | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme A1-II from Escherichia coli, 52.9fold | Sphingomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.4 | - |
purified enzyme | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | isozyme A1-II performs endolytic cleavage of the glycosidic bonds, beta-elimination reaction, and shows a preference for polyguluronate instead of polymannuronate | Sphingomonas sp. | unsaturated algino-oligosaccharides | isozyme A1-II produces mainly tri- and tetrasaccharides | ? |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Sphingomonas sp. |