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Literature summary for 4.2.2.3 extracted from

  • Davidson, I.W.; Lawson, C.J.; Sutherland, I.W.
    An alginate lysate from Azotobacter vinelandii phage (1977), J. Gen. Microbiol., 98, 223-229.
    View publication on PubMed
Search for more literature for 4.2.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.94
-
 alginate bacterial alginate containing 25.5% mannuronic acid, determined on the basis of polymeric mannuronic acid available in the substrate Azotobacter vinelandii phage
1.05
-
 alginate algal alginate containing 31.5% mannuronic acid, determined on the basis of polymeric mannuronic acid available in the substrate Azotobacter vinelandii phage
1.09
-
 alginate bacterial alginate containing 55% mannuronic acid, determined on the basis of polymeric mannuronic acid available in the substrate Azotobacter vinelandii phage
1.28
-
 alginate algal alginate containing 48% mannuronic acid, determined on the basis of polymeric mannuronic acid available in the substrate Azotobacter vinelandii phage

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Azotobacter vinelandii phage
30000 35000
-
 Azotobacter vinelandii phage
50000
-
 gel filtration Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii phage
-
 distinct from the enzyme from the host organism
-

Storage Stability

Storage Stability Organism
-20°C, 3 months, stable Azotobacter vinelandii phage
-20°C, rapid loss of activity Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Azotobacter vinelandii oligouronides
-
 ?
alginate
-
 Azotobacter vinelandii phage oligouronides a series of oligouronides each containing a terminal 4-deoxy-alpha-L-erythrohex-4-enopyranuronosyl residue ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Azotobacter vinelandii phage

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Azotobacter vinelandii phage