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Literature summary for 4.2.2.27 extracted from

  • Pedrolli, D.B.; Carmona, E.C.
    Purification and characterization of a unique pectin lyase from Aspergillus giganteus able to release unsaturated monogalacturonate during pectin degradation (2014), Enzyme Res., 2014, 353915 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.27

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2 mM, 21% inhibition Aspergillus giganteus
EDTA 2 mM, 14% inhibition Aspergillus giganteus
iodoacetic acid 2 mM, 25% inhibition Aspergillus giganteus
PMSF 2 mM, 33% inhibition Aspergillus giganteus
SDS 2 mM, 94% inhibition Aspergillus giganteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for citrus pectin with methylation degree of the carboxyl groups of pectin: 4.9 mg/ml. Km for citrus pectin with methylation degree of the carboxyl groups of pectin: 4.6 mg/ml Aspergillus giganteus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, 1.32fold activation Aspergillus giganteus
Ca2+ 2 mM, 1.07fold activation Aspergillus giganteus
Co2+ the enzyme is stimulated by divalent cations, with Co2+ having the strongest effect. 2 mM, 1.54fold activation Aspergillus giganteus
Mg2+ 2 mM, 1.26fold activation Aspergillus giganteus
NaCl 2 mM, 1.1fold activation Aspergillus giganteus
Pb2+ 2 mM, 1.35fold activation Aspergillus giganteus
Zn2+ 2 mM, 1.17fold activation Aspergillus giganteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Aspergillus giganteus
71000
-
 SDS-PAGE Aspergillus giganteus

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
-
-
Aspergillus giganteus CCT 3232
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus giganteus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-galacturonosyl methyl ester)n the enzyme acts on the non-reducing end of methyl-esterified polygalacturonan, releasing either 4-deoxy-L-threo-hex-4-enopyranuronate or 4-deoxy-6-O-methyl-L-threo-hex-4-enopyranuronate. It is able to act on substrates as short as a disaccharide, and is active on substrates with degrees of methyl esterification ranging between 34% and 90%. The enzyme is able to digest apple pectin and citrus pectins with different degrees of methyl esterification Aspergillus giganteus (1,4-alpha-D-galacturonosyl methyl ester)n-1 + 4-deoxy-6-O-methyl-L-threo-hex-4-enopyranuronate
-
 ?
(1,4-alpha-D-galacturonosyl methyl ester)n the enzyme acts on the non-reducing end of methyl-esterified polygalacturonan, releasing either 4-deoxy-L-threo-hex-4-enopyranuronate or 4-deoxy-6-O-methyl-L-threo-hex-4-enopyranuronate. It is able to act on substrates as short as a disaccharide, and is active on substrates with degrees of methyl esterification ranging between 34% and 90%. The enzyme is able to digest apple pectin and citrus pectins with different degrees of methyl esterification Aspergillus giganteus CCT 3232 (1,4-alpha-D-galacturonosyl methyl ester)n-1 + 4-deoxy-6-O-methyl-L-threo-hex-4-enopyranuronate
-
 ?

Synonyms

Synonyms Comment Organism
exo-pectin lyase
-
 Aspergillus giganteus
PLIII
-
 Aspergillus giganteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus giganteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 35°C: about 50% of maximal activity, 60°C: about 45% of maximal activity Aspergillus giganteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min,in the absence of substrate, 30% loss of activity Aspergillus giganteus
50
-
 half-life: 9 min Aspergillus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Aspergillus giganteus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 45% of maximal activity, pH 9.0: about 55% of maximal activity Aspergillus giganteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 4°C, 24 h, the enzyme solutions keeps more than 90% lyase activity after 24 h Aspergillus giganteus

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme binds an NAD+ cofactor Aspergillus giganteus