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Literature summary for 4.2.2.26 extracted from
- Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase (2016), Biotechnol. Biofuels, 9, 43 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H208F | in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity | Stenotrophomonas maltophilia |
| N207L | completely inactive | Stenotrophomonas maltophilia |
| Y264F | completely inactive | Stenotrophomonas maltophilia |
| Y455F | significantly diminished yet measurable activity toward alginate and M and G blocks | Stenotrophomonas maltophilia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
poly(beta-(1,4)-D-mannuronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia |  |
| 0.41 | - |
poly(beta-(1,4)-D-mannuronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 0.57 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 0.57 | - |
poly(beta-(1,4)glucuronate) | mutant H208F, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 0.67 | - |
alginate | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 0.98 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 1.35 | - |
alginate | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 1.4 | - |
poly(beta-(1,4)glucuronate) | wild-type, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 2.22 | - |
poly(beta-(1,4)guluronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 5.99 | - |
poly(beta-(1,4)guluronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stenotrophomonas maltophilia | B2FSW8 | - |
- |
| Stenotrophomonas maltophilia K279a | B2FSW8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Stenotrophomonas maltophilia | unsaturated algino-monosaccharides | - |
? | |
| alginate | - |
Stenotrophomonas maltophilia K279a | unsaturated algino-monosaccharides | - |
? | |
| poly(beta-(1,4)-D-mannuronate) | - |
Stenotrophomonas maltophilia | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)-D-mannuronate) | - |
Stenotrophomonas maltophilia K279a | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | - |
Stenotrophomonas maltophilia | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | - |
Stenotrophomonas maltophilia K279a | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)glucuronate) | - |
Stenotrophomonas maltophilia | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)glucuronate) | - |
Stenotrophomonas maltophilia K279a | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)guluronate) | - |
Stenotrophomonas maltophilia | 4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid | - |
? | |
| poly(beta-(1,4)guluronate) | - |
Stenotrophomonas maltophilia K279a | 4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Smlt2602 | locus name | Stenotrophomonas maltophilia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
poly(beta-(1,4)glucuronate) | wild-type, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 1.4 | - |
poly(beta-(1,4)guluronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 2 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 6.1 | - |
poly(beta-(1,4)glucuronate) | mutant H208F, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 14.6 | - |
alginate | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 21.3 | - |
poly(beta-(1,4)-D-mannuronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 22.2 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 34.6 | - |
poly(beta-(1,4)guluronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 34.8 | - |
alginate | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 62.2 | - |
poly(beta-(1,4)-D-mannuronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
poly(beta-(1,4)glucuronate) | wild-type, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 0.7 | - |
poly(beta-(1,4)guluronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 2.1 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 5.8 | - |
poly(beta-(1,4)guluronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 10.6 | - |
poly(beta-(1,4)glucuronate) | mutant H208F, pH 8.5, 25°C | Stenotrophomonas maltophilia | |
| 10.8 | - |
alginate | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 38.7 | - |
poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 51.5 | - |
poly(beta-(1,4)-D-mannuronate) | mutant H208F, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 52.2 | - |
alginate | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
| 151.8 | - |
poly(beta-(1,4)-D-mannuronate) | wild-type, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
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