Literature summary for 4.2.2.26 extracted from
Ryu, M.; Lee, E.
Saccharification of alginate by using exolytic oligoalginate lyase from marine bacterium Sphingomonas sp. MJ-3 (2011), J. Ind. Eng. Chem., 17, 853-858.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
saccharification of alginate into alginate monosaccharides. Unsaturated monosaccharides up to 3.3 mg/ml are successfully prepared from 1% (w/v) alginate by using the recombinant oligoalginate lyase of Sphingomonas sp. MJ-3 |
Sphingomonas sp. |
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Escherichia coli |
Sphingomonas sp. |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Cu2+ |
1 mM, 5% residual activity |
Sphingomonas sp. |
|
Hg2+ |
1 mM, no residual activity |
Sphingomonas sp. |
|
Zn2+ |
1 mM, 30% residual activity |
Sphingomonas sp. |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
KCl |
1 mM, 120% of initial activity |
Sphingomonas sp. |
|
NaCl |
1 mM, 115% of initial activity |
Sphingomonas sp. |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
79900 |
- |
x * 79900, calculated |
Sphingomonas sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Sphingomonas sp. |
G1EH67 |
- |
- |
Sphingomonas sp. MJ-3 |
G1EH67 |
- |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
alginate |
- |
Sphingomonas sp. |
unsaturated algino-monosaccharides |
alginate substrate is almost completely depolymerized into monosaccharides |
? |
|
alginate |
- |
Sphingomonas sp. MJ-3 |
unsaturated algino-monosaccharides |
alginate substrate is almost completely depolymerized into monosaccharides |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 79900, calculated |
Sphingomonas sp. |