Any feedback?
Literature summary for 4.2.2.25 extracted from
- Biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of Geobacillus stearothermophilus 98 (2006), Extremophiles, 10, 321-326.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus |  |
| FeCl2 | 1 mM, 1.2fold activation | Geobacillus stearothermophilus | |
| KCl | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
| Li2SO4 | 1 mM, 1.3fold activation | Geobacillus stearothermophilus | |
| MgCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
| MnCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 50% inhibition | Geobacillus stearothermophilus | |
| EDTA | 1 mM, 22% inhibition | Geobacillus stearothermophilus | |
| N-bromosuccinimide | 1 mM, 81% inhibition | Geobacillus stearothermophilus | |
| p-chloromercuribenzoate | 1 mM, 39% inhibition | Geobacillus stearothermophilus | |
| sodium lauryl sulfate | 1 mM, 81% inhibition | Geobacillus stearothermophilus | |
| Urea | 1 mM, 22% inhibition | Geobacillus stearothermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the strain produces thermostable gellan lyase extracellularly during exponential phase | Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
3 * or 4 * 120000, SDS-PAGE | Geobacillus stearothermophilus |
| 350000 | - |
two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis | Geobacillus stearothermophilus |
| 480000 | - |
two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | P85513 | isolated from Bulgarian hot spring | - |
| Geobacillus stearothermophilus 98 | P85513 | isolated from Bulgarian hot spring | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.99 | - |
pH 7.0, 70°C | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gellan | - |
Geobacillus stearothermophilus | ? | - |
? | |
| gellan | - |
Geobacillus stearothermophilus 98 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 3 * or 4 * 120000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
24 h, stable | Geobacillus stearothermophilus |
| 70 | - |
half-life: 50 min in absence of substrate. Stable for 2.5 h in presence of substrate | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Geobacillus stearothermophilus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Geobacillus stearothermophilus | enzyme activity increases tenfold in conditions of continuous cultivation compared to data from batch fermentations and enzyme productivity is almost sixfold higher | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
