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Literature summary for 4.2.2.25 extracted from

  • Miyake, O.; Kobayashi, E.; Nankai, H.; Hashimoto, W.; Mikami, B.; Murata, K.
    Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1 (2004), Arch. Biochem. Biophys., 422, 211-220.
    View publication on PubMed
Search for more literature for 4.2.2.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ significantly inhibits at 0.1 mM Bacillus sp. (in: Bacteria)
additional information Ca2+, Co2+, Cu2+, Fe2+, Mo2+, Mg2+, and Zn2+ show no effect on the enzyme activity at 1 mM. Sulfhydryl agents such as dithiothreitol, glutathione (reduced form), 2-mercaptoethanol, and N-ethylmaleimide (1mM) exhibit no effect on the enzyme activity Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Co2+, Cu2+, Fe2+, Mo2+, Mg2+, and Zn2+ show no effect on the enzyme activity at 1 mM Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 gel filtration Bacillus sp. (in: Bacteria)
259456
-
 1 * 259456, calculated from sequence Bacillus sp. (in: Bacteria)
260000
-
 1 * 260000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) O82833
-
-
Bacillus sp. (in: Bacteria) GL1 O82833
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the 260 kDa enzyme is cleaved between the 1205Gly and 1206Leu residues to yield the mature 130 kDa form of the gellan lyase Bacillus sp. (in: Bacteria)

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gellan highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates Bacillus sp. (in: Bacteria) ?
-
 ?
gellan highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates Bacillus sp. (in: Bacteria) GL1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 259456, calculated from sequence Bacillus sp. (in: Bacteria)
monomer 1 * 260000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 75% of maximal activity, 60°C: about 40% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 7.0, 10 min, more than 50% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 35% of maximal activity, pH 9.0: about 50% of maximal activity Bacillus sp. (in: Bacteria)