Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | significantly inhibits at 0.1 mM | Bacillus sp. (in: Bacteria) | |
additional information | Ca2+, Co2+, Cu2+, Fe2+, Mo2+, Mg2+, and Zn2+ show no effect on the enzyme activity at 1 mM. Sulfhydryl agents such as dithiothreitol, glutathione (reduced form), 2-mercaptoethanol, and N-ethylmaleimide (1mM) exhibit no effect on the enzyme activity | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Co2+, Cu2+, Fe2+, Mo2+, Mg2+, and Zn2+ show no effect on the enzyme activity at 1 mM | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
259456 | - |
1 * 259456, calculated from sequence | Bacillus sp. (in: Bacteria) |
260000 | - |
1 * 260000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | O82833 | - |
- |
Bacillus sp. (in: Bacteria) GL1 | O82833 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the 260 kDa enzyme is cleaved between the 1205Gly and 1206Leu residues to yield the mature 130 kDa form of the gellan lyase | Bacillus sp. (in: Bacteria) |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus sp. (in: Bacteria) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates | Bacillus sp. (in: Bacteria) | ? | - |
? | |
gellan | highly specific to gellan, especially deacetylated gellan, other gellanrelated polysaccharides such as S-88, welan, rhamsan, and S-198 are inert as substrates | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 259456, calculated from sequence | Bacillus sp. (in: Bacteria) |
monomer | 1 * 260000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 75% of maximal activity, 60°C: about 40% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 7.0, 10 min, more than 50% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 35% of maximal activity, pH 9.0: about 50% of maximal activity | Bacillus sp. (in: Bacteria) |