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Literature summary for 4.2.2.25 extracted from

  • Hashimoto, W.; Maesaka, K.; Sato, N.; Kimura, S.; Yamamoto, K.; Kumagai, H.; Murata, K.
    Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1 (1997), Arch. Biochem. Biophys., 339, 17-23.
    View publication on PubMed
Search for more literature for 4.2.2.25

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gellan Bacillus sp. (in: Bacteria) degradation of gellan ?
-
 ?
gellan Bacillus sp. (in: Bacteria) GL1 degradation of gellan ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) O82833
-
-
Bacillus sp. (in: Bacteria) GL1 O82833
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium of the bacterium grown in the presence of gellan as a carbon source Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.34
-
 pH 7.0, 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gellan degradation of gellan Bacillus sp. (in: Bacteria) ?
-
 ?
gellan degradation of gellan Bacillus sp. (in: Bacteria) GL1 ?
-
 ?
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal Bacillus sp. (in: Bacteria) beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1
-
 ?
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal Bacillus sp. (in: Bacteria) GL1 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1
-
 ?

Subunits

Subunits Comment Organism
? x * 140000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus sp. (in: Bacteria)