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Literature summary for 4.2.2.24 extracted from

  • Kunishige, Y.; Iwai, M.; Nakazawa, M.; Ueda, M.; Tada, T.; Nishimura, S.; Sakamoto, T.
    Crystal structure of exo-rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26 (2018), FEBS Lett., 592, 1378-1388 .
    View publication on PubMed
Search for more literature for 4.2.2.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium chrysogenum

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with substrates, to 2.85 A resolution. The substrate-binding site is in the clefts formed by domains II and III and not located near the subunit-interaction surface and the Ca2+-binding site Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
H782A 800fold decrease in activity Penicillium chrysogenum
H782F 650fold decrease in activity Penicillium chrysogenum
Q646A 100fold decrease in activity Penicillium chrysogenum
R634A 10000fold decrease in activity Penicillium chrysogenum
R634L 10000fold decrease in activity Penicillium chrysogenum
R648A 10000fold decrease in activity Penicillium chrysogenum
R648K 10000fold decrease in activity Penicillium chrysogenum
R648L 10000fold decrease in activity Penicillium chrysogenum
Y458A 10000fold decrease in activity Penicillium chrysogenum
Y458F 10000fold decrease in activity Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum A0A0C6EFY4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA
-
 Penicillium chrysogenum ?
-
 ?
beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA
-
 Penicillium chrysogenum ?
-
 ?
beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha
-
 Penicillium chrysogenum ?
-
 ?
beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA
-
 Penicillium chrysogenum ?
-
 ?
additional information enzyme is an exo-type polysaccharide lyase that cleaves rhamnogalacturonan from the reducing end. The active site possesses an L-shaped cleft that can accommodate galactosyl side chains Penicillium chrysogenum ?
-
 ?
unsaturated beta-D-4-deoxy-DELTA4,5-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha
-
 Penicillium chrysogenum ?
-
 ?
unsaturated rhamnogalacturan oligosaccarides
-
 Penicillium chrysogenum 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
 ?

Synonyms

Synonyms Comment Organism
RglX
-
 Penicillium chrysogenum