Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, structure determined at 1.32 A and 1.65 resolution, respectively. YesX crystal belongs to space group P2(1)2(1)2(1) with unit cell parameters of a = 72.9 A, b = 88.1 A, and c = 99.3 A. One molecule is present in an asymmetric unit | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
YesX del_loop | the mutant enzyme shows an endolytic reaction profile. Vmax/Km-value for the mutant enzyme is 275fold lower than the Vmax/KM-value for the wild-type enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
rhamnogalacturonan I | KM-value is 1.78 mg/ml for the wild-type enzyme and 0.56 mg/ml for the, pH 7.5, 30°C | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68754 | - |
x * 68754, calculated from sequence | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I | Bacillus subtilis | responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype reaction of YesX | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31527 | - |
- |
Purification (Comment) | Organism |
---|---|
native YesX and YesX del_loop mutant | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I | responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype reaction of YesX | Bacillus subtilis | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? | |
rhamnogalacturonan I oligosaccharide | exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-GalA bonds of rhamnogalacturonan I oligosacharides containing L-rhamnopyranose at the reducing end and 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end. The products are the disaccharide 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose and the shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | Bacillus subtilis | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68754, calculated from sequence | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
YesX | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |