Literature summary for 4.2.2.23 extracted from

Jensen, M.H.; Otten, H.; Christensen, U.; Borchert, T.V.; Christensen, L.L.; Larsen, S.; Leggio, L.L.
Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus (2010), J. Mol. Biol., 404, 100-111.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion, structure determination of the RGL4_K150A and RGL4_H210A enzyme variants. determination of the 2.4 A crystal structure of the RGL4 mutant RGL4_K150A with an rhamnogalacturonan I hexasaccharide. Crystals of the RGL4_K150A variant soaked with a rhamnogalacturonan digest gave a clear picture of substrate bound in the -3/+3 subsites	Aspergillus aculeatus

Protein Variants

Protein Variants	Comment	Organism
H210A	relative activity is 0.001% of wild-type activity	Aspergillus aculeatus
K150A	relative activity is 0.04% of wild-type activity	Aspergillus aculeatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	rhamnogalacturonan I	Km-value is 1.3 mg/ml or 0.27 mM cleavable bonds, pH 6.0, 30°C	Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
rhamnogalacturonan I	Aspergillus aculeatus	RGL4 is involved in the degradation of rhamnogalacturonan-I, an important pectic plant cell wall polysaccharide	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus aculeatus	Q00019	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
rhamnogalacturonan I	RGL4 is involved in the degradation of rhamnogalacturonan-I, an important pectic plant cell wall polysaccharide	Aspergillus aculeatus	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?
rhamnogalacturonan I	the actual size of the average oligosaccharide is a 25-mer to 30-mer (Mr= 4600 Da), indicating that some of the rhamnogalacturonan regions have a structure recalcitrant to degradation. Domain I of RGL4 contributes the catalytic and many substrate binding residues, but domain III contributes two conserved arginines also involved in substrate binding. Domain II mediates the correct respective positioning of domains I and III	Aspergillus aculeatus	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?

Synonyms

Synonyms	Comment	Organism
RGL4	-	Aspergillus aculeatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	rhamnogalacturonan I	kcat-value is 4.1/s, pH 6.0, 30°C	Aspergillus aculeatus

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Release 2023.1 (January 2023)