Literature summary for 4.2.2.23 extracted from

Pages, S.; Valette, O.; Abdou, L.; Belaich, A.; Belaich, J.P.
A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome (2003), J. Bacteriol., 185, 4727-4733.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Ruminiclostridium cellulolyticum

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	completely abolishes activity against potato pectic galactan	Ruminiclostridium cellulolyticum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	potato pectic galactan	Km-value: 1 g/l, pH 8.5, 37°C	Ruminiclostridium cellulolyticum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cellulosome	Rgl11Y is incorporated in the Clostridium cellulolyticum cellulosome through a typical cohesin-dockerin interaction	Ruminiclostridium cellulolyticum	43263	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required for activity	Ruminiclostridium cellulolyticum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	x * 75000, SDS-PAGE	Ruminiclostridium cellulolyticum

Organism

Organism	UniProt	Comment	Textmining
Ruminiclostridium cellulolyticum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ruminiclostridium cellulolyticum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
potato pectic galactan	beta-eliminative cleavage of the alpha-L-Rhap-(1->4)-alpha-D-GalpA glycosidic bond in the backbone of rhamnogalacturonan I (RGI) via a beta-elimination mechanism. Its specific activity on potato pectic galactan and rhamnogalacturonan is 28 and 3.6 IU/mg, respectively, indicating that Rgl11Y requires galactan decoration of the rhamnogalacturonan backbone	Ruminiclostridium cellulolyticum	potato pectic galactan oligosaccharides	beta-eliminative cleavage of glycosidic bonds with the production of DELTA4,5 unsaturated sugar can be followed spectrophotometrically at 235 nm	?
rhamnogalacturonan I	beta-eliminative cleavage of the alpha-L-Rhap-(1->4)-alpha-D-GalpA glycosidic bond in the backbone of rhamnogalacturonan I (RGI) via a beta-elimination mechanism. Its specific activity on potato pectic galactan and rhamnogalacturonan is 28 and 3.6 IU/mg, respectively, indicating that Rgl11Y requires galactan decoration of the rhamnogalacturonan backbone	Ruminiclostridium cellulolyticum	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	beta-eliminative cleavage of glycosidic bonds with the production of DELTA4,5 unsaturated sugar can be followed spectrophotometrically at 235 nm	?

Subunits

Subunits	Comment	Organism
?	x * 75000, SDS-PAGE	Ruminiclostridium cellulolyticum

Synonyms

Synonyms	Comment	Organism
Rgl11Y	-	Ruminiclostridium cellulolyticum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Ruminiclostridium cellulolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
34	-	potato pectic galactan	pH 8.5, 37°C	Ruminiclostridium cellulolyticum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	substrate: potato pectic galactan	Ruminiclostridium cellulolyticum

pH Range

pH Minimum	pH Maximum	Comment	Organism
10	-	completely inactive at, substrate: potato pectic galactan	Ruminiclostridium cellulolyticum

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Release 2023.1 (January 2023)